Optofluidic Sensor Based on Polymer Optical Microresonators for the Specific, Sensitive and Fast Detection of Chemical and Biochemical Species
Nolwenn-Amandine Keriel,
Camille Delezoide,
David Chauvin,
Hafsa Korri-Youssoufi,
Ngoc Diep Lai,
Isabelle Ledoux-Rak,
Chi-Thanh Nguyen
Affiliations
Nolwenn-Amandine Keriel
Laboratoire Lumière, Matière et Interfaces (LuMIn), Ecole Normale Superieure Paris Saclay, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 9024, CentraleSupelec, Institut d’Alembert, Université Paris Saclay, 4 Avenue des Sciences, 91190 Gif-sur-Yvette, France
Camille Delezoide
Laboratoire Lumière, Matière et Interfaces (LuMIn), Ecole Normale Superieure Paris Saclay, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 9024, CentraleSupelec, Institut d’Alembert, Université Paris Saclay, 4 Avenue des Sciences, 91190 Gif-sur-Yvette, France
David Chauvin
Laboratoire Lumière, Matière et Interfaces (LuMIn), Ecole Normale Superieure Paris Saclay, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 9024, CentraleSupelec, Institut d’Alembert, Université Paris Saclay, 4 Avenue des Sciences, 91190 Gif-sur-Yvette, France
Hafsa Korri-Youssoufi
Institut de Chimie Moléculaire et des Matériaux d’Orsay (ICMMO), Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8182, Université Paris Saclay, 17 Avenue des Sciences, 91400 Orsay, France
Ngoc Diep Lai
Laboratoire Lumière, Matière et Interfaces (LuMIn), Ecole Normale Superieure Paris Saclay, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 9024, CentraleSupelec, Institut d’Alembert, Université Paris Saclay, 4 Avenue des Sciences, 91190 Gif-sur-Yvette, France
Isabelle Ledoux-Rak
Laboratoire Lumière, Matière et Interfaces (LuMIn), Ecole Normale Superieure Paris Saclay, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 9024, CentraleSupelec, Institut d’Alembert, Université Paris Saclay, 4 Avenue des Sciences, 91190 Gif-sur-Yvette, France
Chi-Thanh Nguyen
Laboratoire Lumière, Matière et Interfaces (LuMIn), Ecole Normale Superieure Paris Saclay, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 9024, CentraleSupelec, Institut d’Alembert, Université Paris Saclay, 4 Avenue des Sciences, 91190 Gif-sur-Yvette, France
The accurate, rapid, and specific detection of DNA strands in solution is becoming increasingly important, especially in biomedical applications such as the trace detection of COVID-19 or cancer diagnosis. In this work we present the design, elaboration and characterization of an optofluidic sensor based on a polymer-based microresonator which shows a quick response time, a low detection limit and good sensitivity. The device is composed of a micro-racetrack waveguide vertically coupled to a bus waveguide and embedded within a microfluidic circuit. The spectral response of the microresonator, in air or immersed in deionised water, shows quality factors up to 72,900 and contrasts up to 0.9. The concentration of DNA strands in water is related to the spectral shift of the microresonator transmission function, as measured at the inflection points of resonance peaks in order to optimize the signal-over-noise ratio. After functionalization by a DNA probe strand on the surface of the microresonator, a specific and real time measurement of the complementary DNA strands in the solution is realized. Additionally, we have inferred the dissociation constant value of the binding equilibrium of the two complementary DNA strands and evidenced a sensitivity of 16.0 pm/µM and a detection limit of 121 nM.