PLoS ONE (Jan 2015)

Detection of Peptide-based nanoparticles in blood plasma by ELISA.

  • Gerard H Bode,
  • Karin E Pickl,
  • Maria Sanchez-Purrà,
  • Berta Albaiges,
  • Salvador Borrós,
  • Andy J G Pötgens,
  • Christoph Schmitz,
  • Frank M Sinner,
  • Mario Losen,
  • Harry W M Steinbusch,
  • Hans-Georg Frank,
  • Pilar Martinez-Martinez,
  • European NanoBioPharmaceutics Research Initiative

DOI
https://doi.org/10.1371/journal.pone.0126136
Journal volume & issue
Vol. 10, no. 5
p. e0126136

Abstract

Read online

The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma.A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology.The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl methacrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS.We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.