Nature Communications (May 2017)
A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes
- Qiuhong Li,
- Andrew P. Hutchins,
- Yong Chen,
- Shengbiao Li,
- Yongli Shan,
- Baojian Liao,
- Dejin Zheng,
- Xi Shi,
- Yinxiong Li,
- Wai-Yee Chan,
- Guangjin Pan,
- Shicheng Wei,
- Xiaodong Shu,
- Duanqing Pei
Affiliations
- Qiuhong Li
- CAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
- Andrew P. Hutchins
- CAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
- Yong Chen
- CAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
- Shengbiao Li
- CAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
- Yongli Shan
- CAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
- Baojian Liao
- CAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
- Dejin Zheng
- CAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
- Xi Shi
- CAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
- Yinxiong Li
- CAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
- Wai-Yee Chan
- CAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
- Guangjin Pan
- CAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
- Shicheng Wei
- Central Laboratory, School and Hospital of Stomatology, Peking University
- Xiaodong Shu
- CAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
- Duanqing Pei
- CAS Key laboratory of Regenerative Biology, Guangdong Key laboratory of Stem Cell and Regenerative Medicine and CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health-Guangzhou Medical University Joint School of Biological Sciences, South China Institute of Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
- DOI
- https://doi.org/10.1038/ncomms15166
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 12
Abstract
Reprogramming has been shown to involve EMT-MET; however, its role in cell differentiation is unclear. Here the authors show that duringin vitrodifferentiation of hepatocytes, Activin A-induced formation of definitive endoderm requires EMT mediated by TGFβ signalling, followed by a MET process.
