Chemical and Biochemical Engineering Quarterly (Jan 2019)

Effect of Developed Low Cost Minimal Medium on Lipid and Exopolysaccharide Production by Lipomyces starkeyi Under Repeated Fed-batch and Continuous Cultivation

  • V. Thirumal,
  • A. Chistoserdov,
  • R. Bajpai,
  • J. Bader,
  • M. K. Popovic,
  • R. Subramaniam

DOI
https://doi.org/10.15255/CABEQ.2018.1389
Journal volume & issue
Vol. 32, no. 4
pp. 473 – 481

Abstract

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The main objective of this study was to investigate the effects of a low cost minimal medium, developed by the UL Bioprocessing Lab, on the cultivation of Lipomyces starkeyi NRRL Y-11557 using repeated fed-batch and continuous fermentation strategies. The highest cell and lipid concentrations obtained were 22.7 g L–1 and 11.67 g L–1 under repeated fed-batch cultivation, respectively. Continuous cultivation with the dilution rate of 0.06 h–1 presented the highest cell (0.401 g g–1) and lipid yields (0.177g g–1). Exopolysaccharide production was observed when L. starkeyi was cultivated in the minimal media supplemented with 90 g L–1 glucose under repeated fed-batch fermentation. The produced exopolysaccharide is likely composed of 4–5 repeating sugar units, incorporating mannose and galactose and their respective uronic acids.

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