A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination

Karel Miettinen; Nattawat Leelahakorn; Aldo Almeida; Yong Zhao; Lukas R. Hansen; Iben E. Nikolajsen; Jens B. Andersen; Michael Givskov; Dan Staerk; Søren Bak; Sotirios C. Kampranis

doi:10.1038/s41467-022-31357-6

Nature Communications (Jun 2022)

A GPCR-based yeast biosensor for biomedical, biotechnological, and point-of-use cannabinoid determination

Karel Miettinen,
Nattawat Leelahakorn,
Aldo Almeida,
Yong Zhao,
Lukas R. Hansen,
Iben E. Nikolajsen,
Jens B. Andersen,
Michael Givskov,
Dan Staerk,
Søren Bak,
Sotirios C. Kampranis

Affiliations

Karel Miettinen: Biochemical Engineering Group, Plant Biochemistry Section, Department of Plant and Environmental Sciences, University of Copenhagen
Nattawat Leelahakorn: Biochemical Engineering Group, Plant Biochemistry Section, Department of Plant and Environmental Sciences, University of Copenhagen
Aldo Almeida: Biochemical Engineering Group, Plant Biochemistry Section, Department of Plant and Environmental Sciences, University of Copenhagen
Yong Zhao: Biochemical Engineering Group, Plant Biochemistry Section, Department of Plant and Environmental Sciences, University of Copenhagen
Lukas R. Hansen: Biochemical Engineering Group, Plant Biochemistry Section, Department of Plant and Environmental Sciences, University of Copenhagen
Iben E. Nikolajsen: Biochemical Engineering Group, Plant Biochemistry Section, Department of Plant and Environmental Sciences, University of Copenhagen
Jens B. Andersen: Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen
Michael Givskov: Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen
Dan Staerk: Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen
Søren Bak: Biochemical Engineering Group, Plant Biochemistry Section, Department of Plant and Environmental Sciences, University of Copenhagen
Sotirios C. Kampranis: Biochemical Engineering Group, Plant Biochemistry Section, Department of Plant and Environmental Sciences, University of Copenhagen

DOI: https://doi.org/10.1038/s41467-022-31357-6
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 16

Abstract

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GPCRs are used for diverse sensing in eukaryotes. Here the authors use GPCRs to construct yeast-based biosensors, focussing on cannabinoids, and use these to screen agonists and antagonists, as well as generate a portable detection device.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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