Effect of modified Zhu Jing prescriptions on the expression of VEGF in pathological myopia with choroidal neovascularization

Abstract

AIM: To observe the effect of traditional medicine to the expression of vascular endothelium growth factor(VEGF), and explore the intervention effect to the pathological myopia with choroidal neovascularization(CNV)through experimental model of lasers -induced CNV in guinea pigs with pathological myopia induced by form deprivation. METHODS: Fifteen of the forty-five 3-week old female guinea pigs were randomly selected as the blank control group, the rest 30 guinea pigs were using facemask to induce high myopia in 4 weeks, then randomly assigned to model group and treatment group, then induced CNV by different wavelength laser. The treatment group gavaged with modified Zhu Jing prescriptions while the control group was gavaged with distill water. 21 days after treatment, histopathologic choroidal flat mounts, HE stain,immunofluorescence stain and immuno- histochemistry for VEGF in the experimental eyes and control eyes were performed.RESULTS:All the 30 guinea pigs were successfully induced high myopia and the axial length of the right eyes were significantly greater than the non-facemask eyes and the eyes of control group. Immunofluorescence stain and immunohistochemistry for VEGF showed that the expression of VEGF of the treatment group was less than the model group. The average optical density(AOD)of the form deprivation eye in the treatment group(0.0589±0.0146)was higher than that in the model group(0.0972±0.0507)(PCONCLUSION: Modified Zhu Jing prescriptions has a positive effect to the reduction of the VEGF expression in the CNV with pathological myopia and thus it can inhibit the growth of CNV.

Keywords

• modified Zhu Jing prescriptions
• pathological myopia
• choroidal neovascularization
• vascular endothelium growth factor