Lavandula angustifolia L. plants regeneration from in vitro leaf explants-derived callus as conservation strategy

Abstract

Lavandula angustifolia L. is an aromatic and medicinal herb with multiple industrial applications. Nevertheless, the over exploitation of wild plantation attempts against to its conservation as natural resource. The aim of this paper was to regenerate plants of L. angustifolia from in vitro leaf explants by indirect organogenesis as conservation strategy. Leaves sections from in vitro plants were cultured in MS with 6-Bencylamino purine (BAP) combined with Naphthaleneacetic acid (NAA) or 2,4-Diclophenoxic acetic acid (2,4-D) to induce callus formation. The callus growth was categorized into three types according to the explant area covered by callus and the fresh and dry weigh (mg) per callus were determined. Then, callus were subcultured on MS with BAP and Kinetin combined with NAA or 2,4-D for shoots regeneration. Shoots were cultured in MS with BAP 8.88 µM, indole butyric acid (IBA) 4.92 µM and 2.68µM NAA for rooting. Plantlets were acclimatized and after 50 days of hardening the plants were transferred to the soil. Explants in all treatments formed calli. The higher percentage of callus formation with abundant growth was achieved in MS with 8.88 µM BAP + 5.36 µM NAA (92%). In MS with 4.44 µM BAP + 4.64 µM Kn+ 2.68 µM NAA, 93% of calli regenerating shoots and25-30 multiple shoots were obtained after 63 days of subculture. Shoots developed roots and plants were successfully acclimatized with 93 percentage of surviving. In vitro leaf of L. angustifolia is a suitable explant for plants regeneration.

Keywords

• auxin
• cytokinin
• lavender