In vitro propagation of Pilosocereus sp.

Abstract

This work was carried out with the objective to establish the necessary cultivation conditions for the multiplication in vitro of Pilosocereus sp. Seeds from mature fruits collected under field conditions were used. The effect of three concentration of NaOCl (1.5, 2.0, 2.5%) in the disinfection of the seeds was studied, as well as the influence of three salts concentration Murashige-Skoog, (MS) (25, 50, 75%) on the germination of the same ones. During the multiplication phase the influence of three concentration of bencilaminopurine (1.0, 2.0 y 3.0 mg l-1) in the culture medium was evaluated. The 100% of seeds free of microbial pollutants was achieved when the 1.5% NaOCl during 10 minutes was used. The biggest germination percentage was achieved in a culture medium with 25% of salts MS (88.5%). For the multiplication of plants the best results were reached when 1.0 mg l-1 of 6-BAP was used and a multiplication coefficient of 2.28 units was obtained. The 100% of plants develop root in a simple culture medium Murashige-Skoog (MS) without regulators of growth. During the acclimatization phase the 88.7% of survival was reached. Key words: biodiversity, cactus, micropropagation, threatened species