Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework

Ping Song; Juwen Shen; Dekai Ye; Baijun Dong; Fei Wang; Hao Pei; Jianbang Wang; Jiye Shi; Lihua Wang; Wei Xue; Yiran Huang; Gang Huang; Xiaolei Zuo; Chunhai Fan

doi:10.1038/s41467-020-14664-8

Nature Communications (Feb 2020)

Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework

Ping Song,
Juwen Shen,
Dekai Ye,
Baijun Dong,
Fei Wang,
Hao Pei,
Jianbang Wang,
Jiye Shi,
Lihua Wang,
Wei Xue,
Yiran Huang,
Gang Huang,
Xiaolei Zuo,
Chunhai Fan

Affiliations

Ping Song: Institute of Molecular Medicine, Department of Urology, Department of Nuclear Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University
Juwen Shen: Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University
Dekai Ye: Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Baijun Dong: Institute of Molecular Medicine, Department of Urology, Department of Nuclear Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University
Fei Wang: Frontiers Science Center for Transformative Molecules, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University
Hao Pei: Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University
Jianbang Wang: Frontiers Science Center for Transformative Molecules, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University
Jiye Shi: Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Lihua Wang: Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences
Wei Xue: Institute of Molecular Medicine, Department of Urology, Department of Nuclear Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University
Yiran Huang: Institute of Molecular Medicine, Department of Urology, Department of Nuclear Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University
Gang Huang: Institute of Molecular Medicine, Department of Urology, Department of Nuclear Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University
Xiaolei Zuo: Institute of Molecular Medicine, Department of Urology, Department of Nuclear Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University
Chunhai Fan: Institute of Molecular Medicine, Department of Urology, Department of Nuclear Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University

DOI: https://doi.org/10.1038/s41467-020-14664-8
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

Read online

Tetrahedral DNA framework-enabled bulk enzyme heterojunctions have been used to program biosensor interfaces. Here, the authors use DNA tetrahedrons to tether enzymes of an enzymatic cascade to gold electrodes, hence raising them over the bulk solution, which led to improved kinetics and sensitivity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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