Generation of a DAPK1 knockout first (conditional ready) human embryonic stem cell line (ZSSYe001-A) by CRISPR-Cas9 technology

Cancan Xu; Zhuowei Zhou; Chang Liu; Xinmei Kang; Xiaofen Zhong; Qi Zhang; Yan Xu

Stem Cell Research (Mar 2020)

Generation of a DAPK1 knockout first (conditional ready) human embryonic stem cell line (ZSSYe001-A) by CRISPR-Cas9 technology

Cancan Xu,
Zhuowei Zhou,
Chang Liu,
Xinmei Kang,
Xiaofen Zhong,
Qi Zhang,
Yan Xu

Affiliations

Cancan Xu: Institute of Physical Science and Information Technology, Anhui University, Hefei 230601, China; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
Zhuowei Zhou: Biotherapy Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
Chang Liu: Biotherapy Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
Xinmei Kang: Biotherapy Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
Xiaofen Zhong: CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
Qi Zhang: Biotherapy Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; Corresponding authors.
Yan Xu: Biotherapy Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; Corresponding authors.

Journal volume & issue: Vol. 43

Abstract

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Death-associated protein kinase 1 (DAPK1) is a Ca2+/calmodulin regulated Ser/Thr kinase involved in various cellular processes including cell death, autophagy and inflammation. Its dysregulation has been linked to tumour metastasis, anti-viral responses, Alzheimer's disease and other neurological disorders. To further investigate the role of DAPK1 in these processes, we generated a DAPK1 knockout first (conditional ready) human embryonic stem (hES) cell line in which the endogenous DAPK1 can be easily restored with expression of FLPe. This cell line provides an ideal model to study the role of DAPK1 in human development and various pathologies related to DAPK1 dysregulation in vitro.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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