Dendritic Cells Activate and Mature after Infection with <it>Mycobacterium tuberculosis</it>

BMC Research Notes. 2011;4(1):247 DOI 10.1186/1756-0500-4-247

 

Journal Homepage

Journal Title: BMC Research Notes

ISSN: 1756-0500 (Online)

Publisher: BMC

LCC Subject Category: Medicine | Science: Biology (General) | Science: Science (General)

Country of publisher: United Kingdom

Language of fulltext: English

Full-text formats available: PDF, HTML

 

AUTHORS

Mamo Gezahagne
Mihret Adane
Tafesse Mesfin
Hailu Asrat
Parida Shreemanta

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 15 weeks

 

Abstract | Full Text

<p>Abstract</p> <p>Background</p> <p>Dendritic cells (DCs) can take up an array of different antigens, including microorganisms which they can process and present more effectively than any other antigen presenting cell. However, whether the interaction between the human DC and <it>Mycobacterium tuberculosis </it>represents a defense mechanism by the invaded host, or helping the invader to evade the defense mechanism of the host is still not clearly understood.</p> <p>Findings</p> <p>To analyze the interactions between <it>M. tuberculosis </it>and immune cells, human peripheral blood monocyte-derived immature DCs were infected with <it>M. tuberculosis </it>H37Rv wild type strain and flow cytometry was used to analyse cell surface expression markers. The ability of the <it>M. tuberculosis </it>infected DC to induce T cell proliferation using 5 and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution technique was also investigated. DCs were found to internalize the mycobacteria and show dose dependent infection and necrosis with different multiplicity of infection. Flow cytometry analysis of cell surface expression markers CD40, CD54, CD80, CD83, CD86 and HLA DR in infected DC revealed significant (p < 0.05) up regulation following infection with <it>M. tuberculosis </it>in comparison to immature DC with no stimulation. Lipopolysaccharide (LPS) from <it>Salmonella abortus equi</it>, a known DC maturation agent, was used as a positive control and showed a comparable up regulation of cell surface markers as observed with <it>M. tuberculosis </it>infected DC. It was revealed that the <it>M. tuberculosis </it>infected DC induced T cell proliferation.</p> <p>Conclusion</p> <p>These data clearly demonstrate that <it>M. tuberculosis </it>induces activation and maturation of human monocyte-derived immature DC as well as induces T cell proliferation <it>in vitro</it>.</p>