Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction

Christoph Niemöller; Julius Wehrle; Julian Riba; Rainer Claus; Nathalie Renz; Janika Rhein; Sabine Bleul; Juliane M. Stosch; Justus Duyster; Christoph Plass; Pavlo Lutsik; Daniel B. Lipka; Michael Lübbert; Heiko Becker

doi:10.1038/s42003-021-01661-w

Communications Biology (Feb 2021)

Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction

Christoph Niemöller,
Julius Wehrle,
Julian Riba,
Rainer Claus,
Nathalie Renz,
Janika Rhein,
Sabine Bleul,
Juliane M. Stosch,
Justus Duyster,
Christoph Plass,
Pavlo Lutsik,
Daniel B. Lipka,
Michael Lübbert,
Heiko Becker

Affiliations

Christoph Niemöller: Department of Medicine I, Medical Center - University of Freiburg
Julius Wehrle: Department of Medicine I, Medical Center - University of Freiburg
Julian Riba: Laboratory for MEMS Applications, Department of Microsystems Engineering - IMTEK, University of Freiburg
Rainer Claus: Department of Medicine I, Medical Center - University of Freiburg
Nathalie Renz: Department of Medicine I, Medical Center - University of Freiburg
Janika Rhein: Department of Medicine I, Medical Center - University of Freiburg
Sabine Bleul: Department of Medicine I, Medical Center - University of Freiburg
Juliane M. Stosch: Department of Medicine I, Medical Center - University of Freiburg
Justus Duyster: Department of Medicine I, Medical Center - University of Freiburg
Christoph Plass: Division of Cancer Epigenomics, German Cancer Research Center (DKFZ)
Pavlo Lutsik: Division of Cancer Epigenomics, German Cancer Research Center (DKFZ)
Daniel B. Lipka: Section Translational Cancer Epigenomics, Division of Translational Medical Oncology, German Cancer Research Center (DKFZ) & National Center for Tumor Diseases (NCT) Heidelberg
Michael Lübbert: Department of Medicine I, Medical Center - University of Freiburg
Heiko Becker: Department of Medicine I, Medical Center - University of Freiburg

DOI: https://doi.org/10.1038/s42003-021-01661-w
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 10

Abstract

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Niemöller et al. describe a bisulfite-free assay to assess epigenomics and genomics of single cells (epi-gSCAR). epi-gSCAR generates accurate and reproducible measurements of global DNA methylation and genetic variants at the single cell level, allowing for in-depth analyses of cell-to-cell heterogeneity.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal