International Journal of Nanomedicine (Nov 2024)
Direct Labeling of Gold Nanoparticles with Iodine-131 for Tumor Radionuclide Therapy
Abstract
Meilin Zhu,1 Lingzhou Zhao,2 Xia Lu2 1School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia, 750004, People’s Republic of China; 2Department of Nuclear Medicine, Northern Jiangsu People’s Hospital, Clinical Medical College, Yangzhou University, Yangzhou, 225001, People’s Republic of ChinaCorrespondence: Xia Lu, Department of Nuclear Medicine, Northern Jiangsu People’s Hospital, Clinical Medical College, Yangzhou University, Yangzhou, 225001, People’s Republic of China, Tel +86 18051061486, Email [email protected]: Gold nanoparticles (Au NPs) are widely used as versatile templates to develop multifunctional nanosystems for disease diagnosis and treatment. Iodine can bind to gold via chemisorption, making this a simple method for labeling Au NPs with radioactive iodine. However, the evaluation of tumor radionuclide therapy is insufficient. In this study, we investigated the feasibility of 131I-adsorbed Au NPs as novel nanoprobes for tumor radionuclide therapy.Materials and Methods: Radiolabeling was performed by mixing Au NPs and 131I, and the radiochemical purity (RCP) and in vitro stability of 131I-adsorbed Au NPs were analyzed under different conditions, including various temperatures, pH values, and 131I concentrations. The tumor accumulation and therapeutic potential of 131I-adsorbed Au NPs were assessed using a subcutaneous tumor model after intratumoral injection.Results: The data showed that the chemisorption of the Au NPs onto 131I was instant, specific, and quantitative. The 131I-adsorbed Au NPs exhibited high in vitro stability in different media, distinct inhibitory effects on tumor cells in vitro, good retention ability, and therapeutic effects after intratumoral injection into tumor-bearing mice in vivo.Conclusion: Our work demonstrates that chemisorption of Au NPs and radioiodine has great potential as a strategy for constructing various nanosystems for theranostic applications.Keywords: gold nanoparticles, iodine-131, chemisorption, radionuclide therapy
