Frontiers in Molecular Neuroscience (Oct 2020)
Profiling Microglia From Alzheimer’s Disease Donors and Non-demented Elderly in Acute Human Postmortem Cortical Tissue
- Astrid M. Alsema,
- Qiong Jiang,
- Laura Kracht,
- Emma Gerrits,
- Marissa L. Dubbelaar,
- Anneke Miedema,
- Nieske Brouwer,
- Elly M. Hol,
- Jinte Middeldorp,
- Roland van Dijk,
- Maya Woodbury,
- Astrid Wachter,
- Simon Xi,
- Thomas Möller,
- Knut P. Biber,
- Susanne M. Kooistra,
- Erik W. G. M. Boddeke,
- Erik W. G. M. Boddeke,
- Bart J. L. Eggen
Affiliations
- Astrid M. Alsema
- Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands
- Qiong Jiang
- Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands
- Laura Kracht
- Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands
- Emma Gerrits
- Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands
- Marissa L. Dubbelaar
- Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands
- Anneke Miedema
- Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands
- Nieske Brouwer
- Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands
- Elly M. Hol
- Department of Translational Neuroscience, UMC Utrecht Brain Centre, University Medical Centre Utrecht, University Utrecht, Utrecht, Netherlands
- Jinte Middeldorp
- Department of Translational Neuroscience, UMC Utrecht Brain Centre, University Medical Centre Utrecht, University Utrecht, Utrecht, Netherlands
- Roland van Dijk
- Department of Translational Neuroscience, UMC Utrecht Brain Centre, University Medical Centre Utrecht, University Utrecht, Utrecht, Netherlands
- Maya Woodbury
- Foundational Neuroscience Center, AbbVie Inc., Cambridge, MA, United States
- Astrid Wachter
- Neuroscience Discovery, AbbVie Deutschland GmbH and Co. KG, Ludwigshafen, Germany
- Simon Xi
- Foundational Neuroscience Center, AbbVie Inc., Cambridge, MA, United States
- Thomas Möller
- Foundational Neuroscience Center, AbbVie Inc., Cambridge, MA, United States
- Knut P. Biber
- Neuroscience Discovery, AbbVie Deutschland GmbH and Co. KG, Ludwigshafen, Germany
- Susanne M. Kooistra
- Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands
- Erik W. G. M. Boddeke
- Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands
- Erik W. G. M. Boddeke
- Department of Cellular and Molecular Medicine, Center for Healthy Ageing, University of Copenhagen, Copenhagen, Denmark
- Bart J. L. Eggen
- Department of Biomedical Sciences of Cells and Systems, Section Molecular Neurobiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands
- DOI
- https://doi.org/10.3389/fnmol.2020.00134
- Journal volume & issue
-
Vol. 13
Abstract
Microglia are the tissue-resident macrophages of the central nervous system (CNS). Recent studies based on bulk and single-cell RNA sequencing in mice indicate high relevance of microglia with respect to risk genes and neuro-inflammation in Alzheimer’s disease (AD). Here, we investigated microglia transcriptomes at bulk and single-cell levels in non-demented elderly and AD donors using acute human postmortem cortical brain samples. We identified seven human microglial subpopulations with heterogeneity in gene expression. Notably, gene expression profiles and subcluster composition of microglia did not differ between AD donors and non-demented elderly in bulk RNA sequencing nor in single-cell sequencing.
Keywords
