Generation and characterization of human umbilical cord blood-derived induced pluripotent stem cells (KRIBBi005-A)

Hyebin Koh; Xing Zhen; Jieun Kim; Hye-Yeong Ha; Jong-Hee Lee

Stem Cell Research (Apr 2022)

Generation and characterization of human umbilical cord blood-derived induced pluripotent stem cells (KRIBBi005-A)

Hyebin Koh,
Xing Zhen,
Jieun Kim,
Hye-Yeong Ha,
Jong-Hee Lee

Affiliations

Hyebin Koh: Futuristic Animal Resource & Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea; Department of Functional Genomics, KRIBB School of Bioscience, University of Science and Technology (UST), Daejeon, Republic of Korea
Xing Zhen: National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea; Department of Nanoscience and Nanotechnology, Graduate School, Kyungpook National University, Daegu 41566, Republic of Korea
Jieun Kim: National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea; Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Biomedicine & Health Sciences, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
Hye-Yeong Ha: Division of Intractable Disease Research, Korea National Institute of Health, Cheongju, Republic of Korea
Jong-Hee Lee: National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea; Department of Functional Genomics, KRIBB School of Bioscience, University of Science and Technology (UST), Daejeon, Republic of Korea; Corresponding author.

Journal volume & issue: Vol. 60
p. 102674

Abstract

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Induced pluripotent stem cells (iPSCs) hold great promise as a potential source for regenerative medicine. Umbilical cord blood (UCB) cells possess a high level of stem cells, exhibit less immune rejection, and have fewer DNA mutations and, thus, are an easily accessible and valuable cell source for iPSC generation for basic research and clinical applications. Here, we reprogrammed CD34+-UCB cells with the group O/D-negative (RhD-) blood type as a universal source for red blood cell (RBC) generation. The resulting iPSCs, with features typical of pluripotent stem cells, will further advance the development of various therapeutics, including blood transfusion.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal