Isolation of human cutaneous immune cells for single-cell RNA sequencing

Abstract

Summary: Single-cell RNA sequencing (scRNA-seq) allows for high-resolution analysis of transcriptionally dysregulated cell subpopulations in inflammatory diseases. However, it can be challenging to properly isolate viable immune cells from human skin for scRNA-seq due to its barrier properties. Here, we present a protocol to isolate high-viability human cutaneous immune cells. We describe steps for obtaining and enzymatically dissociating a skin biopsy specimen and isolating immune cells using flow cytometry. We then provide an overview of downstream computational techniques to analyze sequencing data.For complete details on the use and execution of this protocol, please refer to Cook et al. (2022)1 and Liu et al. (2022).2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Cell Isolation
• Flow Cytometry/Mass Cytometry
• Immunology
• Molecular Biology
• RNAseq
• Single Cell