Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering

Carla Schmidt; Paul Fransz; Michelle Rönspies; Steven Dreissig; Jörg Fuchs; Stefan Heckmann; Andreas Houben; Holger Puchta

doi:10.1038/s41467-020-18277-z

Nature Communications (Sep 2020)

Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering

Carla Schmidt,
Paul Fransz,
Michelle Rönspies,
Steven Dreissig,
Jörg Fuchs,
Stefan Heckmann,
Andreas Houben,
Holger Puchta

Affiliations

Carla Schmidt: Botanical Institute, Karlsruhe Institute of Technology
Paul Fransz: Department of Plant Development and (Epi)Genetics, Swammerdam Institute of Life Sciences, University of Amsterdam
Michelle Rönspies: Botanical Institute, Karlsruhe Institute of Technology
Steven Dreissig: Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle Wittenberg
Jörg Fuchs: Leibniz Institute of Plant Genetics and Crop Plant Research
Stefan Heckmann: Leibniz Institute of Plant Genetics and Crop Plant Research
Andreas Houben: Leibniz Institute of Plant Genetics and Crop Plant Research
Holger Puchta: Botanical Institute, Karlsruhe Institute of Technology

DOI: https://doi.org/10.1038/s41467-020-18277-z
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 8

Abstract

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The heterochromatic knob (hk4S) on Arabidopsis chromosome 4 prevents the recombination between accessions with and without hk4S. Here, via egg-cell specific expression of the Cas9 nuclease, the authors demonstrate targeted reversal of the 1.1 Mb long hk4S-inversion in Col-0 and restore the crossovers with Ler-1.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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