Bio-Protocol
(Oct 2016)
Mouse Corneal Stroma Fibroblast Primary Cell Culture
Yujin Zhang,
Yen-Chiao Wang,
Okada Yuka,
Lingling Zhang,
Chia-Yang Liu
Affiliations
Yujin Zhang
School of Optometry, Indiana University, Bloomington, USA
Yen-Chiao Wang
School of Optometry, Indiana University, Bloomington, USA
Okada Yuka
School of Optometry, Indiana University, Bloomington, USADepartment of Ophthalmology, Wakayama Medical University, Wakayama, Japan
Lingling Zhang
School of Optometry, Indiana University, Bloomington, USA
Chia-Yang Liu
School of Optometry, Indiana University, Bloomington, USA
DOI
https://doi.org/10.21769/BioProtoc.1960
Journal volume & issue
Vol. 6,
no. 19
Abstract
Read online
This protocol is developed for primary cell culture of cornea stromal keratocytes isolated from neonatal mouse eyeballs. It provides an optimal condition to isolate stromal keratocytes which maintain high viability for cell culture.
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