Effects of melon yellow spot orthotospovirus infection on the preference and developmental traits of melon thrips, Thrips palmi, in cucumber.

Abstract

Melon yellow spot orthotospovirus (MYSV), a member of the genus Orthotospovirus, is an important virus in cucurbits. Thrips palmi is considered the most serious pest of cucurbits because it directly damages and indirectly transmits MYSV to the plant. The effects of MYSV-infected plants on the development time, fecundity, and preference of the thrips were analyzed in this study. Our results showed that the development time of male and female thrips did not differ significantly between MYSV-infected and non-infected cucumbers. The survival rate of thrips in non-infected and MYSV-infected cucumbers were not significantly different. In a non-choice assay, T. palmi adults were released on non-infected and MYSV-infected cucumbers and allowed to lay eggs. The number of hatched larvae did not significantly differ between non-infected and MYSV-infected cucumbers. In a choice assay, MYSV had no detectable effect on the number of adult thrips and preceding hatched larvae. In a pull assay, the settling rate of thrips on the released plant did not differ significantly when the adult thrips were released to non-infected or MYSV infected cucumbers for any cucumber cultivar. Based on our results, we propose that the effects of MYSV-infected cucumbers on the development time, fecundity, or preference of T. palmi may not be an important factor in MYSV spread between cucumbers.