Acta Cirúrgica Brasileira (Feb 2017)

Amniotic membrane as an option for treatment of acute Achilles tendon injury in rats

  • Mariana de Castro Nicodemo,
  • Lia Renó das Neves,
  • Josafá Carvalho Aguiar,
  • Flaviane de Souza Brito,
  • Isabelle Ferreira,
  • Luciana Barros Sant'Anna,
  • Leandro José Raniero,
  • Rodrigo Álvaro Lopes Martins,
  • Paulo Roxo Barja,
  • Emilia Angela Lo Schiavo Arisawa

DOI
https://doi.org/10.1590/s0102-865020170205
Journal volume & issue
Vol. 32, no. 2
pp. 125 – 139

Abstract

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Abstract Purpose: To evaluate the effect of human amniotic membrane (hAM) fragment on inflammatory response, proliferation of fibroblast and organization of collagen fibers in injured tendon. Methods: Sixty rats were divided into 3 groups: C - surgical procedures without tendon lesion and with simulation of hAM application; I - surgical procedures, tendon injury and simulation of hAM application; T - surgical procedures, tendon injury and hAM application. These groups were subdivided into four experimental times (3, 7, 14 and 28 days). The samples underwent histological analysis and ATR-FTIR spectroscopy. Results: Histological analysis at 14 days, the T group showed collagen fibers with better alignment. At 28 days, the I group presented the characteristics described for the T group at 14 days, while this group presented aspects of a mature connective tissue. FT-IR analysis showed a clear distinction among the three groups at all experimental times and groups T and I presented more similarities to each other than to group C. Conclusion: Acute injury of tendon treated with human amniotic membrane fragment showed a faster healing process, reduction in inflammatory response, intense proliferation of fibroblasts and organization of collagen fibers.

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