Oxidized Perilla and Linseed Oils Induce Neuronal Apoptosis by Caspase-Dependent and -Independent Pathways
Yuki Ueno,
Yoshiyuki Kawamoto,
Yamato Nakane,
Risa Natsume,
Kyoko Miura,
Yui Okumura,
Takashi Murate,
Emi Hattori,
Toshihiko Osawa
Affiliations
Yuki Ueno
Department of Health and Nutrition, Faculty of Psychological and Physical Science, Aichi Gakuin University, 12 Araike Iwasaki-cho, Nisshin, Aichi 470-0195, Japan
Yoshiyuki Kawamoto
Department of Biomedical Sciences, Collage of Life and Health Sciences, Chubu University, Kasugai, Aichi 487-8501, Japan
Yamato Nakane
Department of Health and Nutrition, Faculty of Psychological and Physical Science, Aichi Gakuin University, 12 Araike Iwasaki-cho, Nisshin, Aichi 470-0195, Japan
Risa Natsume
Department of Health and Nutrition, Faculty of Psychological and Physical Science, Aichi Gakuin University, 12 Araike Iwasaki-cho, Nisshin, Aichi 470-0195, Japan
Kyoko Miura
Department of Health and Nutrition, Faculty of Psychological and Physical Science, Aichi Gakuin University, 12 Araike Iwasaki-cho, Nisshin, Aichi 470-0195, Japan
Yui Okumura
Department of Biomedical Sciences, Collage of Life and Health Sciences, Chubu University, Kasugai, Aichi 487-8501, Japan
Takashi Murate
Department of Biomedical Sciences, Collage of Life and Health Sciences, Chubu University, Kasugai, Aichi 487-8501, Japan
Emi Hattori
Department of Health and Nutrition, Faculty of Psychological and Physical Science, Aichi Gakuin University, 12 Araike Iwasaki-cho, Nisshin, Aichi 470-0195, Japan
Toshihiko Osawa
Department of Health and Nutrition, Faculty of Psychological and Physical Science, Aichi Gakuin University, 12 Araike Iwasaki-cho, Nisshin, Aichi 470-0195, Japan
Alpha-linolenic acid (ALA), a polyunsaturated fatty acid, is involved in bioregulatory functions. In recent years, the health-promoting effects of vegetable-derived edible oils rich in ALA have attracted attention. ALA has a variety of physiological effects such as anti-arteriosclerotic and antiallergic properties, but is prone to oxidation. Therefore, safety concerns exist with regard to adverse effects on humans induced by its oxides. However, the effects on neuronal cells induced by oxidized ALA-rich oils, such as perilla and linseed oils, have not been fully investigated. This information is very important from the viewpoint of food safety. In this study, we investigated the effects of oxidized perilla and linseed oils, which are rich in ALA, on the toxicity of neuronal SH-SY5Y cells. Perilla and linseed oils were significantly oxidized compared with other edible vegetable oils. These oxidized oils induce neuronal cell death and apoptosis via caspase-dependent and -independent pathways through reactive oxygen species (ROS) generation. Furthermore, they suppressed neurite outgrowth. These results suggest that oxidized perilla and linseed oils have the potential to cause neuronal loss and ROS-mediated apoptosis, and thus may affect the onset and progression of neurodegenerative disorders and other diseases.
