Improved Joint Health Following Oral Administration of Glycosaminoglycans with Native Type II Collagen in a Rabbit Model of Osteoarthritis
Vicente Sifre,
Carme Soler,
Sergi Segarra,
José Ignacio Redondo,
Luis Doménech,
Amadeo Ten-Esteve,
Laura Vilalta,
Luis Pardo-Marín,
Claudio Iván Serra
Affiliations
Vicente Sifre
Programa de Doctorado en Ciencias de la Vida y del Medio Natural, Escuela de Doctorado, Universidad Católica de Valencia San Vicente Mártir, 46002 Valencia, Spain
Carme Soler
Hospital Veterinario UCV, Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia San Vicente Mártir, 46002 Valencia, Spain
Sergi Segarra
R&D Bioiberica S.A.U., 08950 Esplugues de Llobregat, Spain
José Ignacio Redondo
Departamento Medicina y Cirugía Animal, Universidad Cardenal Herrera-CEU, CEU Universities, 46115 Valencia, Spain
Luis Doménech
Departamento de Matemáticas, Física y Ciencias Tecnológicas, Universidad Cardenal Herrera-CEU, CEU Universities, 46115 Valencia, Spain
Amadeo Ten-Esteve
Biomedical Imaging Research Group (GIBI230-PREBI), La Fe Health Research Institute, and Imaging La Fe node at Distributed Network for Biomedical Imaging (ReDIB) Unique Scientific and Technical Infrastructures (ICTS), 46026 Valencia, Spain
Laura Vilalta
Hospital Veterinario UCV, Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia San Vicente Mártir, 46002 Valencia, Spain
Luis Pardo-Marín
Interlab-UMU, Campus de Excelencia “Mare Nostrum”, University of Murcia, Campus Espinardo, 30071 Murcia, Spain
Claudio Iván Serra
Hospital Veterinario UCV, Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria y Ciencias Experimentales, Universidad Católica de Valencia San Vicente Mártir, 46002 Valencia, Spain
A prospective, experimental, randomized, double blinded study was designed to evaluate the effects of glycosaminoglycans, with or without native type II collagen (NC), in an osteoarthritis model induced by cranial cruciate ligament transection. The following compounds were tested: chondroitin sulfate (CS), glucosamine hydrochloride (GlHCl), hyaluronic acid (HA) and NC. Fifty-four female 12-week-old New Zealand rabbits were classified into three groups: CTR (control–no treatment), CGH (CS + GlHCl + HA) and CGH-NC (CS + GlHCl + HA + NC). Each group was subdivided into three subgroups according to survival times of 24, 56 and 84 days. Over time, all rabbits developed degenerative changes associated with osteoarthritis. CGH-NC showed significantly improved values on macroscopic evaluation, compared to CTR and CGH. Microscopically, significantly better results were seen with CGH and CGH-NC, compared to CTR, and synovial membrane values were significantly better with CGH-NC compared to CGH. A significant improvement in magnetic resonance imaging biomarkers was also observed with CGH-NC in cartilage transversal relaxation time (T2) and subchondral bone D2D fractal dimension in the lateral condyle. In conclusion, our results show beneficial effects on joint health of CGH and CGH-NC and also supports that adding NC to CGH results in even greater efficacy.
