Nanobody-based RFP-dependent Cre recombinase for selective anterograde tracing in RFP-expressing transgenic animals

Ayumu Inutsuka; Sho Maejima; Hiroyuki Mizoguchi; Ryosuke Kaneko; Rei Nomura; Keiko Takanami; Hirotaka Sakamoto; Tatsushi Onaka

doi:10.1038/s42003-022-03944-2

Communications Biology (Sep 2022)

Nanobody-based RFP-dependent Cre recombinase for selective anterograde tracing in RFP-expressing transgenic animals

Ayumu Inutsuka,
Sho Maejima,
Hiroyuki Mizoguchi,
Ryosuke Kaneko,
Rei Nomura,
Keiko Takanami,
Hirotaka Sakamoto,
Tatsushi Onaka

Affiliations

Ayumu Inutsuka: Division of Brain and Neurophysiology, Department of Physiology, Jichi Medical University
Sho Maejima: Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University, Ushimado
Hiroyuki Mizoguchi: Department of Neuropsychopharmacology and Hospital Pharmacy, Nagoya University Graduate School of Medicine
Ryosuke Kaneko: KOKORO-Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences, Osaka University
Rei Nomura: Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University, Ushimado
Keiko Takanami: Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University, Ushimado
Hirotaka Sakamoto: Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University, Ushimado
Tatsushi Onaka: Division of Brain and Neurophysiology, Department of Physiology, Jichi Medical University

DOI: https://doi.org/10.1038/s42003-022-03944-2
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 13

Abstract

Read online

A Cre recombinase dependent on red fluorescent protein (RFP) is generated, which expands the repertory of nanobody-based genetic tools by enabling selective targeting of RFP-dependent gene expression in the mouse and rat brain.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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