Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota
Jin-Yi Wan,
Jing-Xuan Wan,
Shilei Wang,
Xiaolu Wang,
Wenqian Guo,
Han Ma,
Yuqi Wu,
Chong-Zhi Wang,
Lian-Wen Qi,
Ping Li,
Haiqiang Yao,
Chun-Su Yuan
Affiliations
Jin-Yi Wan
School of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, China
Jing-Xuan Wan
School of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, China
Shilei Wang
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China
Xiaolu Wang
School of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, China
Wenqian Guo
School of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, China
Han Ma
School of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, China
Yuqi Wu
School of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, China
Chong-Zhi Wang
Tang Center for Herbal Medicine Research & Department of Anesthesia and Critical Care, University of Chicago, Chicago, IL, USA
Lian-Wen Qi
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China
Ping Li
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China
Haiqiang Yao
School of Traditional Chinese Medicine & National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, China
Chun-Su Yuan
Tang Center for Herbal Medicine Research & Department of Anesthesia and Critical Care, University of Chicago, Chicago, IL, USA
Oplopanax elatus (Nakai) Nakai, in the Araliaceae family, has been used in traditional Chinese medicine (TCM) to treat diseases as an adaptogen for thousands of years. This study established an ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS) method to identify chemical components and biotransformation metabolites of root bark extract from O. elatus. A total of 18 compounds were characterized in O. elatus extract, and 62 metabolites by human intestinal microbiota were detected. Two polyynes, falcarindiol and oplopandiol were recognized as the main components of O. elatus, whose metabolites are further illustrated. Several metabolic pathways were proposed to generate the detected metabolites, including methylation, hydrogenation, demethylation, dehydroxylation, and hydroxylation. These findings indicated that intestinal microbiota might play an essential role in mediating the bioactivity of O. elatus.
