Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells
Kevin O’Rourke,
Sarah Ackerman,
Lukas Dow,
Scott Lowe
Affiliations
Kevin O’Rourke
Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program, New York, USACancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, USA
Sarah Ackerman
Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, USA
Lukas Dow
Department of Medicine, Weill Cornell Medical College, New York, USA
Scott Lowe
Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, USAHoward Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, New York, USA
In this protocol we describe our modifications to a method to isolate, culture and maintain mouse intestinal stem cells as crypt-villus forming organoids. These cells, isolated either from the small or large intestine, maintain self-renewal and multilineage differentiation potential over time. This provides investigators a tool to culture wild type or transformed intestinal epithelium, and a robust assay for stem cell tissue homeostasis in vitro.
