Use of GnRH for Synchronization of the Follicular Wave in Assisted Reproductive Technologies in Sheep: A Preliminary Study
Aina Año-Perello,
Zurisaday Santos-Jimenez,
Teresa Encinas,
Paula Martinez-Ros,
Antonio Gonzalez-Bulnes
Affiliations
Aina Año-Perello
Departamento de Produccion y Sanidad Animal, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, C/ Tirant lo Blanc, 7. 46115 Alfara del Patriarca Valencia, Spain
Zurisaday Santos-Jimenez
Departamento Ciencias Medico Veterinarias, Universidad Autónoma Agraria Antonio Narro, Torreón, 25315 Coahuila, Mexico
Teresa Encinas
Departamento de Farmacologia y Toxicologia Facultad de Veterinaria, UCM, Ciudad Universitaria s/n, 28040 Madrid, Spain
Paula Martinez-Ros
Departamento de Produccion y Sanidad Animal, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, C/ Tirant lo Blanc, 7. 46115 Alfara del Patriarca Valencia, Spain
Antonio Gonzalez-Bulnes
Departamento de Produccion y Sanidad Animal, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, C/ Tirant lo Blanc, 7. 46115 Alfara del Patriarca Valencia, Spain
The present study aimed to set up a short-term protocol for synchronization of follicular wave emergence in sheep, concomitant with estrus synchronization, which would improve ovarian response in assisted reproductive technologies. Administration of a single GnRH dose, concomitant with the insertion of a progesterone-loaded CIDR device, caused regression of gonadotrophin-dependent follicles ≥4 mm in all the GnRH-treated sheep and in around 80% of the controls treated only with CIDR (p p < 0.05). The remaining sheep showed follicles that were still not dependent of luteinizing hormone (LH). So, in fact, all the sheep had non-dominant follicles after treatment. In conclusion, a treatment including GnRH at CIDR insertion would offer a time- and cost-efficient protocol for inducing follicular turnover and synchronizing a new follicular wave at any stage of the estrous cycle.
