Bergenin Activates SIRT1 as a Novel Therapeutic Agent for Osteogenesis of Bone Mesenchymal Stem Cells

Weiduo Hou; Weiduo Hou; Chenyi Ye; Chenyi Ye; Mo Chen; Weixu Li; Weixu Li; Xiang Gao; Xiang Gao; Rongxin He; Rongxin He; Qiang Zheng; Qiang Zheng; Wei Zhang; Wei Zhang

doi:10.3389/fphar.2019.00618

Frontiers in Pharmacology (Jun 2019)

Bergenin Activates SIRT1 as a Novel Therapeutic Agent for Osteogenesis of Bone Mesenchymal Stem Cells

Weiduo Hou,
Weiduo Hou,
Chenyi Ye,
Chenyi Ye,
Mo Chen,
Weixu Li,
Weixu Li,
Xiang Gao,
Xiang Gao,
Rongxin He,
Rongxin He,
Qiang Zheng,
Qiang Zheng,
Wei Zhang,
Wei Zhang

Affiliations

Weiduo Hou: Department of Orthopedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
Weiduo Hou: Research institute of Orthopaedics, Zhejiang University, Hangzhou, China
Chenyi Ye: Department of Orthopedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
Chenyi Ye: Research institute of Orthopaedics, Zhejiang University, Hangzhou, China
Mo Chen: Department of Rheumatology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
Weixu Li: Department of Orthopedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
Weixu Li: Research institute of Orthopaedics, Zhejiang University, Hangzhou, China
Xiang Gao: Department of Orthopedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
Xiang Gao: Research institute of Orthopaedics, Zhejiang University, Hangzhou, China
Rongxin He: Department of Orthopedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
Rongxin He: Research institute of Orthopaedics, Zhejiang University, Hangzhou, China
Qiang Zheng: Department of Orthopedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
Qiang Zheng: Research institute of Orthopaedics, Zhejiang University, Hangzhou, China
Wei Zhang: Department of Orthopedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
Wei Zhang: Research institute of Orthopaedics, Zhejiang University, Hangzhou, China

DOI: https://doi.org/10.3389/fphar.2019.00618
Journal volume & issue: Vol. 10

Abstract

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Bone mesenchymal stem cells (BMSCs) are important candidates for bone regeneration. The role of Bergenin, a C-glucoside of 4-O-methyl gallic acid obtained from the species, Bergenia, in BMSC osteogenesis has not yet been elucidated. We therefore investigated the effects of Bergenin on the osteogenesis of BMSCs and found that Bergenin enhanced osteoblast-specific markers and downregulated the adipocyte-specific markers in vitro. Furthermore, using a rat calvarial defect model, we found that Bergenin significantly improved bone healing, as determined by imaging and histological analyses. Moreover, it also upregulated SIRT1 expression. A SIRT1 inhibitor (EX 527) decreased the enhanced bone mineral formation caused by Bergenin. Taken together, these findings show that Bergenin accelerated the osteogenic differentiation of BMSCs, at least partly through the activation of SIRT1.

Published in Frontiers in Pharmacology

ISSN: 1663-9812 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://journal.frontiersin.org/journals/pharmacology

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