Effect of Inhibiting LITAF Expression on Proliferation, Apoptosis and Radiosensitivity of Human Glioblastoma U251 Cell

Abstract

Objective To investigate the expression of LITAF in human glioblastoma tissues and the effect of LITAF silence on the proliferation, apoptosis and radiosensitivity of glioblastoma U251 cells. Methods The expression of LITAF in glioblastoma was analyzed in The Cancer Genome Atlas (TCGA) database. The proliferation and apoptosis of U251 cells were detected with EDU kit and flow cytometry after the expression of LITAF was silenced. Clonogenic assay and flow cytometry were performed to assess the radiosensitivity of U251 cells with LITAF RNAi. Results The expression of LITAF was increased significantly in human glioblastoma tissues, compared with normal brain tissues. The proliferation and apoptosis of U251 cells weren' t interfered in U251 cells after LITAF knockdown by RNAi. After irradiation, the apoptosis was decreased remarkably and the clone formation was increased markedly in U251 cells with LITAF RNAi, compared with the control cells. Conclusion LITAF is highly expressed in human glioblastoma tissues. Knockdown of LITAF expression has no effect on the proliferation or apoptosis but decreases the radiosensitivity of U251 cells.

Keywords

• huma glioblastoma
• litaf
• u251 cells
• proliferation
• apoptosis
• radiosensitivity