Evaluation of Periodontal Ligament Fibroblast Viability in 0.2% Hyaluronic Acid–containing Gel as a Storage Medium for Preserving of Avulsed Teeth: An In Vitro Study

Abstract

Introduction: We aimed to evaluate the effect of a 0.2% hyaluronic acid–containing gel (Gengigel) on periodontal ligament fibroblast viability in avulsed teeth. Methods: One hundred freshly extracted teeth were collected. The samples were divided into three experimental groups: Gengigel, Hank’s balanced salt solution, and a Dry group. The teeth were immediately immersed in the media and subdivided into three subgroups (10 for each) according to the time intervals: 1, 4, and 8 hours. Cells were examined using a light microscope and a hemocytometer after being labeled with Trypan blue. Results: After 1 hour, the difference between study groups was insignificant (P = 2.203). After 4 and 8 hours, the difference between study groups was significant (P < 0.001). The pairwise post-hoc test revealed significant differences among all study groups at both the 4- (P ≤ 0.002) and 8-hour (P < 0.001) time intervals. Conclusion: Gengigel may serve as an alternative transport medium for preserving fibroblast viability in avulsed teeth.

Keywords

• periodontal ligament fibroblast
• cell viability
• hyaluronic acid
• avulsed teeth
• tooth injuries
• transport media