A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells

Xiaohua Ma; Guoguang Wu; Yuehua Zhao; Zibo Yuan; Yu Zhang; Ning Xia; Mengnan Yang; Lin Liu

doi:10.1155/2018/1986468

Journal of Analytical Methods in Chemistry (Jan 2018)

A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells

Xiaohua Ma,
Guoguang Wu,
Yuehua Zhao,
Zibo Yuan,
Yu Zhang,
Ning Xia,
Mengnan Yang,
Lin Liu

Affiliations

Xiaohua Ma: School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou, Jiangsu 221116, China
Guoguang Wu: School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou, Jiangsu 221116, China
Yuehua Zhao: Key Laboratory of New Optoelectronic Functional Materials (Henan Province), College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, China
Zibo Yuan: Key Laboratory of New Optoelectronic Functional Materials (Henan Province), College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, China
Yu Zhang: Key Laboratory of New Optoelectronic Functional Materials (Henan Province), College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, China
Ning Xia: Key Laboratory of New Optoelectronic Functional Materials (Henan Province), College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, China
Mengnan Yang: Key Laboratory of New Optoelectronic Functional Materials (Henan Province), College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, China
Lin Liu: Key Laboratory of New Optoelectronic Functional Materials (Henan Province), College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan 455000, China

DOI: https://doi.org/10.1155/2018/1986468
Journal volume & issue: Vol. 2018

Abstract

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We reported here a turn-on fluorescent probe (1) for the detection of cysteine (Cys) by incorporating the recognition unit of 2,4-dinitrobenzenesulfonyl ester (DNBS) to a coumarin derivative. The structure of the obtained probe was confirmed by NMR and HRMS techniques. The probe shows a remarkable fluorescence off-on response (∼52-fold) by the reaction with Cys in 100% aqueous buffer. The sensing mechanism was verified by the HPLC test. Probe 1 also displays high selectivity towards Cys. The detection limit was calculated to be 23 nM. Moreover, cellular experiments demonstrated that the probe is highly biocompatible and can be used for monitoring intracellular Cys.

Published in Journal of Analytical Methods in Chemistry

ISSN: 2090-8865 (Print); 2090-8873 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Science: Chemistry: Analytical chemistry
Website: https://onlinelibrary.wiley.com/journal/1471

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