LSAMP-AS1 binds to microRNA-183–5p to suppress the progression of prostate cancer by up-regulating the tumor suppressor DCN
Xing Hua,
Zhen Liu,
Min Zhou,
Yan Tian,
Pei-Pei Zhao,
Wen-Hai Pan,
Chao-Xia Li,
Xiao-Xiao Huang,
Ze-Xiao Liao,
Qi Xian,
Bo Chen,
Yue Hu,
Lei Leng,
Xiao-Wei Fang,
Li-Na Yu
Affiliations
Xing Hua
Departments of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou 510220, P.R. China
Zhen Liu
Department of Pathology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, P.R. China
Min Zhou
Department of Otolaryngology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510006, P.R. China
Yan Tian
Department of pathology, School of Basic Medical Sciences, Southern Medical University, Guanghou 510515, P.R.China; Department of pathology, Nanfang Hospital, Guanghou 510515, P.R. China; Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, P.R. China
Pei-Pei Zhao
Department of pathology, School of Basic Medical Sciences, Southern Medical University, Guanghou 510515, P.R.China; Department of pathology, Nanfang Hospital, Guanghou 510515, P.R. China; Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, P.R. China
Wen-Hai Pan
Departments of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou 510220, P.R. China
Chao-Xia Li
Departments of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou 510220, P.R. China
Xiao-Xiao Huang
Departments of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou 510220, P.R. China
Ze-Xiao Liao
Departments of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou 510220, P.R. China
Qi Xian
Departments of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou 510220, P.R. China
Bo Chen
Departments of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou 510220, P.R. China
Yue Hu
Departments of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou 510220, P.R. China
Lei Leng
Departments of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou 510220, P.R. China
Xiao-Wei Fang
Departments of Pathology, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou 510220, P.R. China
Li-Na Yu
Department of pathology, School of Basic Medical Sciences, Southern Medical University, Guanghou 510515, P.R.China; Department of pathology, Nanfang Hospital, Guanghou 510515, P.R. China; Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, P.R. China; Correspondence to: Dr. Li-Na Yu, Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guanghou, No. 1838, North.
Background: : Prostate cancer (PCa) is a leading cause of cancer-related death in males. Aberrant expression of long noncoding RNAs (lncRNAs) is frequently reported in human malignancies. This study was performed to explore the role of LSAMP-AS1 in epithelial-mesenchymal transition (EMT), proliferation, migration and invasion of PCa cells. Methods: : Initially, the differentially expressed lncRNAs in PCa were screened out by microarray analysis. The clinicopathological and prognostic significance of LSAMP-AS1 was evaluated. LSAMP-AS1 was over-expressed or silenced to investigate the roles in EMT, proliferation, migration and invasion of PCa cells. Moreover, the relationships between LSAMP-AS1 and miR-183–5p, as well as miR-183–5p and decorin (DCN) were characterized. The tumorigenicity of PCa cells was verified in nude mice. Results: : LSAMP-AS1 was poorly expressed in PCa tissues and cells. Low expression of LSAMP-AS1 was indicative of poor overall survival and disease-free survival, and related to Gleason score, TNM stage, and risk stratification. Over-expressed LSAMP-AS1 inhibited EMT, proliferation, migration and invasion of PCa cells, as well as tumor growth in nude mice. Meanwhile, over-expression of LSAMP-AS1 resulted in up-regulation of E-cadherin and down-regulation of Vimentin, N-cadherin, Ki67, PCNA, MMP-2, MMP-9, Ezrin and Fascin. Notably, LSAMP-AS1 competitively bound to miR-183–5p which directly targets DCN. It was confirmed that the inhibitory effect of LSAMP-AS1 on PCa cells was achieved by binding to miR-183–5p, thus promoting the expression of DCN. Conclusion: : LSAMP-AS1 up-regulates the DCN gene by competitively binding to miR-183–5p, thus inhibiting EMT, proliferation, migration and invasion of PCa cells. Keywords: Prostate cancer, LSAMP-AS1, MicroRNA-183–5p, Decorin, Epithelial-mesenchymal transition, Proliferation, Migration
