VISUAL PATTERNS OF С4А AND C4B SUBISOTYPES OF C4 COMPONENT OF HUMAN COMPLEMENT SYSTEM IN THE DIAGNOSTICS OF AUTOIMMUNE AND INFECTIOUS DISEASES

Abstract

Results on visual patterns of subisotypes of patient sera complement component C4 complexes separated by isoelectric focusing in polyacrylamide gel and eiectrobiotting are presented. Method of visual controlled desialylation of sera using iso types C4A and C4B as internal biomarkers is described. Method was applied to investigation C4 isotyping and subisotyping patient sera. Sera of patients were classified into groups differing in subisotyping. The number of established groups of system component C4 was higher compared to groups detecting by other methods (for example, detecting using microplate functional assay of isotypes C4A and С4В]. Results open new prospects in diagnostics of autoimmune and infectious diseases using detection of pathological blood biomarkers complexed to C4 isotypes.

Keywords

• complement system
• isotypes c4a and c4b
• desialylation of serum
• autoimmune and infectious diseases
• diagnostics