Fine-mapping and candidate gene identification for QPtn.sau-4B showing potential in increasing productive tiller number and yield in wheat
Jiajun Liu,
Tongzhu Wang,
Yuxin Lan,
Zhiyuan Zhang,
Jianing You,
Lei Wu,
Xinrong Hu,
Li Yin,
Yanlin Liu,
Huaping Tang,
Qiantao Jiang,
Qiang Xu,
Guoyue Chen,
Pengfei Qi,
Yunfeng Jiang,
Yuming Wei,
Youliang Zheng,
Chunji Liu,
Jian Ma
Affiliations
Jiajun Liu
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Dazhou Key Laboratory of Agricultural Resources Development and Ecological Conservation in Daba Mountain/Research Center of Sichuan Old Revolutionary Areas Development, Sichuan University of Arts and Science, Dazhou 635000, Sichuan, China
Tongzhu Wang
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Yuxin Lan
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Zhiyuan Zhang
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Jianing You
Industrial Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610300, Sichuan, China
Lei Wu
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Xinrong Hu
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Li Yin
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Yanlin Liu
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Huaping Tang
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Qiantao Jiang
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Qiang Xu
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Guoyue Chen
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Pengfei Qi
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Yunfeng Jiang
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Yuming Wei
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Youliang Zheng
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Chunji Liu
School of Agriculture, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China
Jian Ma
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Corresponding author.
Productive tiller number (PTN) is a pivotal trait that significantly influences wheat grain yield. To date, there have been limited reports on the cloning of genes that regulate PTN in wheat. The quantitative trait locus (QTL) QPtn.sau-4B, associated with PTN, was previously mapped between the markers KASP-1 and KASP-3 on the chromosome 4B. Here, utilizing 12 newly developed markers and phenotypic data of PTN from recombinants identified within this interval, QPtn.sau-4B was further fine-mapped to a 2.58 Mb interval on wheat chromosome arm 4BS. Within this interval, we identified 14 genes with high-confidence and 32 genes with low-confidence. A 0.17 Mb deletion fragment contained TraesCS4B03G0092600 and TraesCS4B03G0093100, which were assigned as candidate genes for QPtn.sau-4B. Additionally, QPtn.sau-4B had potential to enhance both PTN and grain yield in wheat. Cloning this locus would support the development of wheat cultivars with increased grain yield.
