Exploring Antioxidant and Enzymes (A-Amylase and B-Glucosidase) Inhibitory Activity of <i>Morinda lucida</i> and <i>Momordica charantia</i> Leaves from Benin
Michaelle Chokki,
Mihaela Cudălbeanu,
Cheikna Zongo,
Durand Dah-Nouvlessounon,
Ioana Otilia Ghinea,
Bianca Furdui,
Robert Raclea,
Aly Savadogo,
Lamine Baba-Moussa,
Sorin Marius Avamescu,
Rodica Mihaela Dinica,
Farid Baba-Moussa
Affiliations
Michaelle Chokki
Laboratoire de Microbiologie et de Technologie Alimentaire, FAST, Université d’Abomey-Calavi, ISBA-Champ de foire, Cotonou 01BP: 526, Benin
Mihaela Cudălbeanu
Department of Chemistry, Physics and Environment, “Dunarea de Jos” University of Galati, 47 Domneasca Street, 800008 Galati, Romania
Cheikna Zongo
Laboratoire de Biochimie et immunologie Appliquées (LABIA), Ecole Doctorale Sciences et Technologies, Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina-Faso
Durand Dah-Nouvlessounon
Department of Chemistry, Physics and Environment, “Dunarea de Jos” University of Galati, 47 Domneasca Street, 800008 Galati, Romania
Ioana Otilia Ghinea
Department of Chemistry, Physics and Environment, “Dunarea de Jos” University of Galati, 47 Domneasca Street, 800008 Galati, Romania
Bianca Furdui
Department of Chemistry, Physics and Environment, “Dunarea de Jos” University of Galati, 47 Domneasca Street, 800008 Galati, Romania
Robert Raclea
Department of Chemistry, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK
Aly Savadogo
Laboratoire de Biochimie et immunologie Appliquées (LABIA), Ecole Doctorale Sciences et Technologies, Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina-Faso
Lamine Baba-Moussa
Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Faculty of Sciences and Techniques, Cotonou 05BP1604, Benin
Sorin Marius Avamescu
Department of Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, 90-92 Soseaua Panduri, Bucharest, Romania
Rodica Mihaela Dinica
Department of Chemistry, Physics and Environment, “Dunarea de Jos” University of Galati, 47 Domneasca Street, 800008 Galati, Romania
Farid Baba-Moussa
Laboratoire de Microbiologie et de Technologie Alimentaire, FAST, Université d’Abomey-Calavi, ISBA-Champ de foire, Cotonou 01BP: 526, Benin
Background: Momordica charantia Linn. (Cucurbitaceae), the wild variety of bitter melon and Morinda lucida Benth (Rubiaceae) were commonly used as a popular folk medicine in Benin. This research focused to measure the antioxidant and enzyme inhibitory effects of M. charantia and M. lucida leaves and their antidiabetic activity. Methods: Antioxidant activities were evaluated by micro-dilution technique using DPPH free radical scavenging activity and β-carotene-linoleate bleaching assay. The α-amylase inhibition assay was carried out utilizing the 3,5-dinitrosalicylic acid procedure, while β-glucosidase inhibition assay was demonstrated using as substrate p-nitrophenyl-β-D-glucopyranoside (PNPG). HPLC-DAD analysis was realized using a high-performance liquid chromatography systems with diode-array detector, L-3000. Results: Chlorogenic acid, epicatechin, daidzein, rutin, naringin, quercetin, naringenin and genistein were identified as polyphenol compounds in the both plants extract. Dichloromethane and ethyl acetate extracts showed a good α-amylase inhibitory activity (56.46 ± 1.96% and 58.76 ± 2.74% respectively). M. lucida methanolic extract has shown IC50 of 0.51 ± 0.01 mg/mL, which is the lowest for DPPH scavenging activity. M. lucida dichloromethane extract showed the highest inhibitory capacity of β-glucosidase activity (82.11. ± 2.15%). Conclusion: These results justify some traditional medicinal uses of both plants. The purified fractions could be used in future formulations, possibly incorporated in functional foods to combat certain diseases.
