New Insights into Cutaneous Asepsis: Synergism between Pfaffia and Rosemary Extracts
Diego Garcia Miranda,
Florence Carrouel,
Tatiane Cristina Alberti Silva,
Mariana Cafalchio Rozzatto,
Amjad Abu Hasna,
Carlos Eduardo Rocha Santos,
Flavia Villaça Morais,
Luciane Dias de Oliveira,
Lucas de Paula Ramos
Affiliations
Diego Garcia Miranda
Gynecology and Obstetrics Service, Woman-Mother-Child Hospital, Hospices Civils de Lyon, 59 Boulevard Pinel, 69500 Lyon, France
Florence Carrouel
Laboratory Health Systemic Process (P2S), UR4129, Faculty of Medicine Laenenc, University Claude Bernard Lyon 1, University of Lyon, 11 Rue Guillaume Paradin, 69008 Lyon, France
Tatiane Cristina Alberti Silva
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Francisco José Longo 777, São José dos Campos 12245-000, São Paulo, Brazil
Mariana Cafalchio Rozzatto
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Francisco José Longo 777, São José dos Campos 12245-000, São Paulo, Brazil
Amjad Abu Hasna
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Francisco José Longo 777, São José dos Campos 12245-000, São Paulo, Brazil
Carlos Eduardo Rocha Santos
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Francisco José Longo 777, São José dos Campos 12245-000, São Paulo, Brazil
Flavia Villaça Morais
Department of Health Sciences, Paulista University, Highway President Dutra, São José dos Campos 12240-420, São Paulo, Brazil
Luciane Dias de Oliveira
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Francisco José Longo 777, São José dos Campos 12245-000, São Paulo, Brazil
Lucas de Paula Ramos
Laboratory Health Systemic Process (P2S), UR4129, Faculty of Medicine Laenenc, University Claude Bernard Lyon 1, University of Lyon, 11 Rue Guillaume Paradin, 69008 Lyon, France
(1) Background: In hospitals, medical and dental clinics, antiseptics or disinfectants play an essential role in the control of nosocomial infections. This study aimed to evaluate R. officinalis and P. paniculata glycolic extracts regarding: (I) their antimicrobial action on planktonic and biofilm (monotypic and cutaneous biofilm model—S. aureus, S. epidermidis and C. acnes); and (II) their cytotoxicity on human keratinocytes (HaCaT). (2) Methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were performed (CLSI protocol M7-A6 and M11-A8). MTT analysis was used to evaluate the antibiofilm activity of the extracts on biofilms and their cytotoxicity on human keratinocytes. (3) Results: The combined glycolic extracts MIX A (75% P. paniculata + 25% R. officinalis); MIX B (50% P. paniculata + 50% R. officinalis); and MIX C (25% P. paniculata + 75% R. officinalis) promoted MBC values by 50 mg/mL on S. aureus, absent on S. epidermidis, and ranged 6.25–50 mg/mL for C. acnes. The cutaneous biofilm model was reduced more than 90%. In addition, it showed biocompatibility with human keratinocytes, resulting in percentages of viability greater than 50%. (4) Conclusions: The combination of extracts promoted antimicrobial action on planktonic cultures, and monotypic and heterotypic biofilms of skin pathogens. Additionally, these extracts are biocompatible against human keratinocytes.
