Nature Communications (Oct 2017)
Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations
- Jérôme Quérard,
- Ruikang Zhang,
- Zsolt Kelemen,
- Marie-Aude Plamont,
- Xiaojiang Xie,
- Raja Chouket,
- Insa Roemgens,
- Yulia Korepina,
- Samantha Albright,
- Eliane Ipendey,
- Michel Volovitch,
- Hanna L. Sladitschek,
- Pierre Neveu,
- Lionel Gissot,
- Arnaud Gautier,
- Jean-Denis Faure,
- Vincent Croquette,
- Thomas Le Saux,
- Ludovic Jullien
Affiliations
- Jérôme Quérard
- PASTEUR, Département de Chimie, École Normale Supérieure, UPMC Univ Paris 06, CNRS, PSL Research University
- Ruikang Zhang
- PASTEUR, Département de Chimie, École Normale Supérieure, UPMC Univ Paris 06, CNRS, PSL Research University
- Zsolt Kelemen
- Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Saclay Plant Sciences (SPS), Universite Paris-Saclay
- Marie-Aude Plamont
- PASTEUR, Département de Chimie, École Normale Supérieure, UPMC Univ Paris 06, CNRS, PSL Research University
- Xiaojiang Xie
- PASTEUR, Département de Chimie, École Normale Supérieure, UPMC Univ Paris 06, CNRS, PSL Research University
- Raja Chouket
- PASTEUR, Département de Chimie, École Normale Supérieure, UPMC Univ Paris 06, CNRS, PSL Research University
- Insa Roemgens
- PASTEUR, Département de Chimie, École Normale Supérieure, UPMC Univ Paris 06, CNRS, PSL Research University
- Yulia Korepina
- PASTEUR, Département de Chimie, École Normale Supérieure, UPMC Univ Paris 06, CNRS, PSL Research University
- Samantha Albright
- PASTEUR, Département de Chimie, École Normale Supérieure, UPMC Univ Paris 06, CNRS, PSL Research University
- Eliane Ipendey
- PASTEUR, Département de Chimie, École Normale Supérieure, UPMC Univ Paris 06, CNRS, PSL Research University
- Michel Volovitch
- Centre for Interdisciplinary Research in Biology (CIRB) CNRS, INSERM, Labex MemoLife, PSL Research University, Collège de France
- Hanna L. Sladitschek
- Cell Biology and Biophysics Unit, European Molecular Biology Laboratory
- Pierre Neveu
- Cell Biology and Biophysics Unit, European Molecular Biology Laboratory
- Lionel Gissot
- Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Saclay Plant Sciences (SPS), Universite Paris-Saclay
- Arnaud Gautier
- PASTEUR, Département de Chimie, École Normale Supérieure, UPMC Univ Paris 06, CNRS, PSL Research University
- Jean-Denis Faure
- Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Saclay Plant Sciences (SPS), Universite Paris-Saclay
- Vincent Croquette
- Ecole Normale Supérieure, Département de Physique and Département de Biologie, Laboratoire de Physique Statistique, CNRS, ENS
- Thomas Le Saux
- PASTEUR, Département de Chimie, École Normale Supérieure, UPMC Univ Paris 06, CNRS, PSL Research University
- Ludovic Jullien
- PASTEUR, Département de Chimie, École Normale Supérieure, UPMC Univ Paris 06, CNRS, PSL Research University
- DOI
- https://doi.org/10.1038/s41467-017-00847-3
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 8
Abstract
Generally, fluorescence imaging needs to be done in a dark environment using molecules with spectrally separated emissions. Here, Quérard et al. develop a protocol for high-speed imaging and remote sensing of spectrally overlapping reversible photoswitchable fluorophores in ambient light.
