DNA profiling of Streptococcus mutans in children with and without black tooth stains: A polymerase chain reaction analysis

Dental Research Journal. 2018;15(5):334-339 DOI 10.4103/1735-3327.240472

 

Journal Homepage

Journal Title: Dental Research Journal

ISSN: 1735-3327 (Print); 2008-0255 (Online)

Publisher: Wolters Kluwer Medknow Publications

Society/Institution: Isfahan University of Medical Sciences

LCC Subject Category: Medicine: Dentistry

Country of publisher: India

Language of fulltext: English

Full-text formats available: PDF, HTML, ePUB

 

AUTHORS

Dhanalakshmi Ravikumar
Deepa Gurunathan
R Gayathri
V Vishnu Priya
R V Geetha

EDITORIAL INFORMATION

Double blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 52 weeks

 

Abstract | Full Text

Background: The purpose of this study was to identify the genetic patterns of Streptococcus mutans by DNA finger printing among caries-active, caries-free, and in children with black tooth stains. Materials and Methods: In this in vitro study Ten children with black tooth stains and ten caries-free and caries-active children between 3 and 6 years of age were recruited. Saliva samples were collected using a sterile tube, followed by microbial culture of S. mutans, DNA isolation, and polymerase chain reaction amplification. The molecular weights of each band were converted into binary data, and data were entered into SPSS to generate similarity dendrograms. Results: Dendrogram interpretation of black tooth stain and caries-free samples revealed an identical genetic pattern in 15 samples with high genetic similarity. Dendrogram interpretation of black tooth stain and caries-active samples exhibited a greater genetic diversity. Conclusion: This study concludes that identical genotypes and high genetic similarity among isolates of the caries-free and black tooth stains. Polymorphism and genetic diversity exist between the black tooth stain and caries-active samples.