Peptide fusion improves prime editing efficiency

Minja Velimirovic; Larissa C. Zanetti; Max W. Shen; James D. Fife; Lin Lin; Minsun Cha; Ersin Akinci; Danielle Barnum; Tian Yu; Richard I. Sherwood

doi:10.1038/s41467-022-31270-y

Nature Communications (Jun 2022)

Peptide fusion improves prime editing efficiency

Minja Velimirovic,
Larissa C. Zanetti,
Max W. Shen,
James D. Fife,
Lin Lin,
Minsun Cha,
Ersin Akinci,
Danielle Barnum,
Tian Yu,
Richard I. Sherwood

Affiliations

Minja Velimirovic: Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School
Larissa C. Zanetti: Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School
Max W. Shen: Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT
James D. Fife: Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School
Lin Lin: Hubrecht Institute
Minsun Cha: Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School
Ersin Akinci: Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School
Danielle Barnum: Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School
Tian Yu: Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School
Richard I. Sherwood: Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School

DOI: https://doi.org/10.1038/s41467-022-31270-y
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 8

Abstract

Read online

Prime editing enables search-and-replace genome editing but is limited by low editing efficiency. Here the authors present PepSEq, a high-throughput method for screening a large library of peptides that influence prime editing efficiency.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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