Some Special Aspects of Liver Repair after Resection and Administration of Multipotent Stromal Cells in Experiment
Igor Maiborodin,
Elena Lushnikova,
Marina Klinnikova,
Swetlana Klochkova
Affiliations
Igor Maiborodin
Laboratory of Cell Biology and Cytology, Institute of Molecular Pathology and Pathomorphology, Federal State Budget Scientific Institution “Federal Research Center of Fundamental and Translational Medicine” of the Ministry of Science and Higher Education of the Russian Federation, Acad. Timakov st., 2, 630117 Novosibirsk, Russia
Elena Lushnikova
Laboratory of Cell Biology and Cytology, Institute of Molecular Pathology and Pathomorphology, Federal State Budget Scientific Institution “Federal Research Center of Fundamental and Translational Medicine” of the Ministry of Science and Higher Education of the Russian Federation, Acad. Timakov st., 2, 630117 Novosibirsk, Russia
Marina Klinnikova
Laboratory of Cell Biology and Cytology, Institute of Molecular Pathology and Pathomorphology, Federal State Budget Scientific Institution “Federal Research Center of Fundamental and Translational Medicine” of the Ministry of Science and Higher Education of the Russian Federation, Acad. Timakov st., 2, 630117 Novosibirsk, Russia
Swetlana Klochkova
Department of Human Anatomy, Peoples Friendship University of Russia (RUDN University), Miklukho-Maklaya st., 117198 Moscow, Russia
Changes in rat liver after resection and injection of autologous multipotent mesenchymal stromal cells of bone marrow origin (MSCs) transfected with the GFP gene and cell membranes stained with red-fluorescent lipophilic membrane dye were studied by light microscopy. It was found that after the introduction of MSCs into the damaged liver, their differentiation into any cells was not found. However, under the conditions of MSCs use, the number of neutrophils in the parenchyma normalizes earlier, and necrosis and hemorrhages disappear more quickly. It was concluded that the use of MSCs at liver resection for the rapid restoration of an organ is inappropriate, since the injected cells in vivo do not differentiate either into hepatocytes, into epithelial cells of bile capillaries, into endotheliocytes and pericytes of the vascular membranes, into fibroblasts of the scar or other connective tissue structures, or into any other cells present in the liver.
