Molecular Oncology (Aug 2022)

Human papilloma virus integration sites and genomic signatures in head and neck squamous cell carcinoma

  • Juliette Mainguené,
  • Sophie Vacher,
  • Maud Kamal,
  • Abderaouf Hamza,
  • Julien Masliah‐Planchon,
  • Sylvain Baulande,
  • Sabrina Ibadioune,
  • Edith Borcoman,
  • Wulfran Cacheux,
  • Valentin Calugaru,
  • Laura Courtois,
  • Carole Crozes,
  • Marc Deloger,
  • Elodie Girard,
  • Jean‐Pierre Delord,
  • Antoine Dubray‐Vautrin,
  • Linda Larbi Chérif,
  • Celia Dupain,
  • Emmanuelle Jeannot,
  • Jerzy Klijanienko,
  • Sonia Lameiras,
  • Charlotte Lecerf,
  • Anouchka Modesto,
  • Alain Nicolas,
  • Roman Rouzier,
  • Esma Saada‐Bouzid,
  • Pierre Saintigny,
  • Anne Sudaka,
  • Nicolas Servant,
  • Christophe Le Tourneau,
  • Ivan Bièche

DOI
https://doi.org/10.1002/1878-0261.13219
Journal volume & issue
Vol. 16, no. 16
pp. 3001 – 3016

Abstract

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A prevalence of around 26% of human papillomavirus (HPV) in head and neck squamous cell carcinoma (HNSCC) has been previously reported. HPV induced oncogenesis mainly involving E6 and E7 viral oncoproteins. In some cases, HPV viral DNA has been detected to integrate with the host genome and possibly contributes to carcinogenesis by affecting the gene expression. We retrospectively assessed HPV integration sites and signatures in 80 HPV positive patients with HNSCC, by using a double capture‐HPV method followed by next‐generation Sequencing. We detected HPV16 in 90% of the analyzed cohort and confirmed five previously described mechanistic signatures of HPV integration [episomal (EPI), integrated in a truncated form revealing two HPV‐chromosomal junctions colinear (2J‐COL) or nonlinear (2J‐NL), multiple hybrid junctions clustering in a single chromosomal region (MJ‐CL) or scattered over different chromosomal regions (MJ‐SC) of the human genome]. Our results suggested that HPV remained episomal in 38.8% of the cases or was integrated/mixed in the remaining 61.2% of patients with HNSCC. We showed a lack of association of HPV genomic signatures to tumour and patient characteristics, as well as patient survival. Similar to other HPV associated cancers, low HPV copy number was associated with worse prognosis. We identified 267 HPV‐human junctions scattered on most chromosomes. Remarkably, we observed four recurrent integration regions: PDL1/PDL2/PLGRKT (8.2%), MYC/PVT1 (6.1%), MACROD2 (4.1%) and KLF5/KLF12 regions (4.1%). We detected the overexpression of PDL1 and MYC upon integration by gene expression analysis. In conclusion, we identified recurrent targeting of several cancer genes such as PDL1 and MYC upon HPV integration, suggesting a role of altered gene expression by HPV integration during HNSCC carcinogenesis.

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