Transcriptome Sequence Analysis of Defense Response of Resistant and Susceptible Bottle Gourd to Powdery Mildew

Zhaohui Zhang; Kuanhong Wang; Chunhong Chen; Shoubo Tian; Jue Wu; Jianyong Li; Lingjuan Kong; Xiaofeng Yang; Changwei Zhang; Ying Li; Hongfang Zhu; Dong Xiao

doi:10.3390/agronomy13051406

Agronomy (May 2023)

Transcriptome Sequence Analysis of Defense Response of Resistant and Susceptible Bottle Gourd to Powdery Mildew

Zhaohui Zhang,
Kuanhong Wang,
Chunhong Chen,
Shoubo Tian,
Jue Wu,
Jianyong Li,
Lingjuan Kong,
Xiaofeng Yang,
Changwei Zhang,
Ying Li,
Hongfang Zhu,
Dong Xiao

Affiliations

Zhaohui Zhang: Shanghai Academy of Agricultural Sciences, Zhuanghang Comprehensive Experiment Station, Shanghai Key Laboratory of Facility Horticulture Technology, Shanghai 201403, China
Kuanhong Wang: Shanghai Academy of Agricultural Sciences, Zhuanghang Comprehensive Experiment Station, Shanghai Key Laboratory of Facility Horticulture Technology, Shanghai 201403, China
Chunhong Chen: Shanghai Academy of Agricultural Sciences, Zhuanghang Comprehensive Experiment Station, Shanghai Key Laboratory of Facility Horticulture Technology, Shanghai 201403, China
Shoubo Tian: The Protected Horticulture Institute, Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai 201403, China
Jue Wu: Fengxian District Agricultural Technology Extension Center, Shanghai 201400, China
Jianyong Li: Shanghai Agricultural Technology Extension and Service Center, Shanghai 201103, China
Lingjuan Kong: Shanghai Agricultural Technology Extension and Service Center, Shanghai 201103, China
Xiaofeng Yang: Shanghai Academy of Agricultural Sciences, Zhuanghang Comprehensive Experiment Station, Shanghai Key Laboratory of Facility Horticulture Technology, Shanghai 201403, China
Changwei Zhang: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Ying Li: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Hongfang Zhu: The Protected Horticulture Institute, Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai 201403, China
Dong Xiao: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

DOI: https://doi.org/10.3390/agronomy13051406
Journal volume & issue: Vol. 13, no. 5
p. 1406

Abstract

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Powdery mildew (PM) is the main disease that afflicts bottle gourd. Previous studies on PM mainly focused on its effects on pumpkin, melon, and other crops; however, the exact molecular mechanism of bottle gourd resistance to PM remains unclear. RNA sequencing (RNA-Seq) technology was used to investigate the dynamic changes in leaf transcriptome profiles between resistant and susceptible gourd at 12, 24, 48, and 72 h post-inoculation with powdery mildew. Compared with a susceptible variety (G3), the expression levels of the differentially expressed genes of phenylpropanoid biosynthesis, starch, and sucrose metabolism, and plant–pathogen interaction pathways in disease-resistant plants were upregulated. We propose that disease resistance and tolerance in bottle gourd are enhanced via several pathways, including the antioxidant system, phenylalanine biosynthesis, and cell wall cellulose synthesis. Our research will provide an important basis for further screening and breeding PM resistance in bottle gourd.

Published in Agronomy

ISSN: 2073-4395 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture
Website: http://www.mdpi.com/journal/agronomy

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