Transcriptome Sequence Analysis of Defense Response of Resistant and Susceptible Bottle Gourd to Powdery Mildew
Zhaohui Zhang,
Kuanhong Wang,
Chunhong Chen,
Shoubo Tian,
Jue Wu,
Jianyong Li,
Lingjuan Kong,
Xiaofeng Yang,
Changwei Zhang,
Ying Li,
Hongfang Zhu,
Dong Xiao
Affiliations
Zhaohui Zhang
Shanghai Academy of Agricultural Sciences, Zhuanghang Comprehensive Experiment Station, Shanghai Key Laboratory of Facility Horticulture Technology, Shanghai 201403, China
Kuanhong Wang
Shanghai Academy of Agricultural Sciences, Zhuanghang Comprehensive Experiment Station, Shanghai Key Laboratory of Facility Horticulture Technology, Shanghai 201403, China
Chunhong Chen
Shanghai Academy of Agricultural Sciences, Zhuanghang Comprehensive Experiment Station, Shanghai Key Laboratory of Facility Horticulture Technology, Shanghai 201403, China
Shoubo Tian
The Protected Horticulture Institute, Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai 201403, China
Jue Wu
Fengxian District Agricultural Technology Extension Center, Shanghai 201400, China
Jianyong Li
Shanghai Agricultural Technology Extension and Service Center, Shanghai 201103, China
Lingjuan Kong
Shanghai Agricultural Technology Extension and Service Center, Shanghai 201103, China
Xiaofeng Yang
Shanghai Academy of Agricultural Sciences, Zhuanghang Comprehensive Experiment Station, Shanghai Key Laboratory of Facility Horticulture Technology, Shanghai 201403, China
Changwei Zhang
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Ying Li
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Hongfang Zhu
The Protected Horticulture Institute, Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai 201403, China
Dong Xiao
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Powdery mildew (PM) is the main disease that afflicts bottle gourd. Previous studies on PM mainly focused on its effects on pumpkin, melon, and other crops; however, the exact molecular mechanism of bottle gourd resistance to PM remains unclear. RNA sequencing (RNA-Seq) technology was used to investigate the dynamic changes in leaf transcriptome profiles between resistant and susceptible gourd at 12, 24, 48, and 72 h post-inoculation with powdery mildew. Compared with a susceptible variety (G3), the expression levels of the differentially expressed genes of phenylpropanoid biosynthesis, starch, and sucrose metabolism, and plant–pathogen interaction pathways in disease-resistant plants were upregulated. We propose that disease resistance and tolerance in bottle gourd are enhanced via several pathways, including the antioxidant system, phenylalanine biosynthesis, and cell wall cellulose synthesis. Our research will provide an important basis for further screening and breeding PM resistance in bottle gourd.
