Establishment of in vitro protocol and impact of mycorrhization with phosphobacteria on micro propagated Pogostemon mollis Benth. (Lamiaceae)

Abstract

Plants and microbes compete for survival and their organized interactions play a vital role in adapting to stress environments. Microbes have a positive impact on transplanting performance of in vitro propagated plantlets. The present study aims at survival development of micropropagated Pogostemon mollis through microbial inoculation. The leaf and stem explants on MS medium with different concentrations and combinations of BAP, Kn, NAA, IAA and IBA used for the establishment of plantlet. The survival rate of plantlets was successfully increased from 71% to about 70.21% by the inoculation of Rhizophagus fasciculatus and Bacillus megaterium. This inoculation also clearly increases the growth, biomass and chlorophyll of in vitro derived platelets. The present protocol emphasizes the need of bio-hardening on micropropagated plants, particularly the mycorrhization along with phosphobacteria.

Keywords

• Pogostemon mollis
• microbial inoculation
• Rhizophagus fasciculatus
• Bacillus megaterium
• micropropagation
• transplanting