OncoTargets and Therapy (Feb 2020)
LncRNA LINC01140 Inhibits Glioma Cell Migration and Invasion via Modulation of miR-199a-3p/ZHX1 Axis
Abstract
Yanchao Xin,1 Wuzhong Zhang,1 Chongchong Mao,2 Jianxin Li,1 Xianzhi Liu,2 Junbo Zhao,1 Junfeng Xue,1 Junqing Li,1 Yonglu Ren1 1Department of Neurosurgery, People’s Hospital of Jiaozuo City, Jiaozuo City, Henan Province 454002, People’s Republic of China; 2Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University in Henan Province, Zhengzhou City, Henan Province 450052, People’s Republic of ChinaCorrespondence: Xianzhi LiuDepartment of Neurosurgery, The First Affiliated Hospital of Zhengzhou University in Henan Province, No. 1 Jianshe East Road, Zhengzhou City, Henan Province 450052, People’s Republic of ChinaTel +86 371-66278517Email [email protected]: Glioma is an aggressive tumor from the nervous system, which causes more than 70% of primary malignant brain tumors. Considering its severe malignancy, there is an urgent need to investigate more practical markers to understand the pathogenesis of glioma, and potential treatment methods for glioma patients. In the paper, we are focused on examining the roles of LINC01140, miR-199a-3p, and ZHX1 in the progression of gliomas, as well as their inner associations and modulation mechanisms.Methods: qRT-PCR was employed to examine the expression levels of LINC01140 and miR-199a-3p. We measured the expressions of ZHX1 via qRT-PCR and Western blotting. CCK8 assays, migration assays, and invasion assays were carried out to determine the cell viabilities and abilities of migration and invasion. We also conducted in vivo tumor growth experiments to investigate the roles of LINC01140 in glioma developments.Results: The expressions of LINC01140 were promoted in glioma. Silencing LINC01140 could inhibit glioma cell viabilities, migration, and invasion. In our experiments, miR-199a-3p was inhibited in glioma. LINC01140 negatively regulated the expressions of miR-199a-3p in glioma. MiR-199a-3p could target ZHX1 to inhibit its expression in glioma cells.Conclusion: LINC01140 could promote glioma developments by modulating the miR-199a-3p/ZHX1 axis.Keywords: LINC01140, glioma, ZHX1, miR-199a-3p
