Dry environment on the expression of lacrimal gland S100A9, Anxa1, and Clu in rats via proteomics

Yi-Lin Sun; A-Yuan Cui; Li-Xin Wang; Wang-Wang Zhang; Hong Shi

doi:10.18240/ijo.2024.03.04

International Journal of Ophthalmology (Mar 2024)

Dry environment on the expression of lacrimal gland S100A9, Anxa1, and Clu in rats via proteomics

Yi-Lin Sun,
A-Yuan Cui,
Li-Xin Wang,
Wang-Wang Zhang,
Hong Shi

Affiliations

Yi-Lin Sun: Hong Shi. College of Traditional Chinese Medicine, Xinjiang Medical University; Research on High Incidence of Diseases in Xinjiang Region, Key Laboratory of the Ministry of Education (Xinjiang Medical University), Urumqi 830011, Xinjiang Uygur Autonomous Region, China. [email protected]
A-Yuan Cui: College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
Li-Xin Wang: College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
Wang-Wang Zhang: College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
Hong Shi: College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; Research on High Incidence of Diseases in Xinjiang Region, Key Laboratory of the Ministry of Education (Xinjiang Medical University), Urumqi 830011, Xinjiang Uygur Autonomous Region, China

DOI: https://doi.org/10.18240/ijo.2024.03.04
Journal volume & issue: Vol. 17, no. 3
pp. 435 – 443

Abstract

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AIM: To investigate the underlying mechanism of dry environment (autumn dryness) affecting the lacrimal glands in rats. METHODS: Twenty Sprague-Dawley rats were randomly divided into two groups. The rats were fed in specific pathogen free environment as the control group (n=10), and the rats fed in dry environment as the dryness group (n=10). After 24d, lacrimal glands were collected from the rats. The tissues morphology was observed by hematoxylin-eosin (HE) staining. Tandem mass tags (TMT) quantitative proteomics analysis technology was used to screen the differential expressed proteins of lacrimal glands between the two groups, then bioinformatics analysis was performed. Further, the immunohistochemical (IHC) method was used to verify the target proteins. RESULTS: In dryness group, the lacrimal glands lobule atrophied, the glandular cavities enlarged, the sparse nuclear distribution and scattered inflammatory infiltration between the acinus were observed. The proteomics exhibited that a total of 195 up-regulated and 236 down-regulated differential expressed proteins screened from the lacrimal glands of rats. It was indicated that the biological processes (BP) of differential expressed proteins mainly included cell processes and single BP. The cellular compositions of differential expressed proteins mainly located in cells, organelles. The molecular functions of differential expressed proteins mainly included binding, catalytic activity. Moreover, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the differential expressed proteins mainly involved lysosome, complement and coagulation cascade, and ribosome pathway. The IHC result verified that the up-regulated expression proteins of Protein S100A9 (S100A9), Annexin A1 (Anxa1), and Clusterin (Clu) in lacrimal glands of rats in dryness group were higher than control group. CONCLUSION: The up-regulated expression proteins of S100A9, Anxa1, and Clu may be the potential mechanisms of dry eye symptoms caused by dry environment. This study provides clues of dry environments causing eye-related diseases for further studies.

Published in International Journal of Ophthalmology

ISSN: 2222-3959 (Print); 2227-4898 (Online)
Publisher: Press of International Journal of Ophthalmology (IJO PRESS)
Country of publisher: China
LCC subjects: Medicine: Ophthalmology
Website: http://www.ijo.cn/gjyken/ch/index.aspx

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