PCR-based markers for identification of some allelic variation at Glu-1 and Glu-3 loci in common wheat

Abstract

Marker assisted selection (MAS) is a tool for breeding, screening, and genetic characterization of germplasm. Allelic variation of both high and low molecular weight glutenin subunits (HMW/LMW-GS) is associated with the rheological properties of wheat flour. In this study, we investigated glutenin pattern using SDS-PAGE and their PCR based on DNA markers in 60 advanced wheat lines and cultivars with different origins. Specific DNA markers regarding to Glu-1 loci, such as 1319 bp, 669 bp and 450 bp fragments were respectively validated for 2*, 17+18, 5+10 alleles. These alleles showed the highest allelic percentage in Glu-1 loci in studied cultivars. However the Null, 7+8 and 5+10 alleles showed the highest allelic percentage in advanced lines. In this study, 23%, 40% and 37% of cultivars respectively, got good (10), moderate (8-9) and weak (4-7) quality scores. In advanced lines, 18%, 44% and 38% got good, moderate and weak quality scores respectively. Ten specific DNA PCR markers were also detected for genotyping Glu-B3 alleles. The most frequent Glu-B3 alleles in wheat cultivars were i, a, b and d with 24%, 21%, 20% and 12%, respectively.Specific PCR markers regarding to the reported Glu-B3 alleles were produced as 621bp, 1095bp, 1570 bp and 662bp consequently. The most frequent Glu-B3 alleles in advanced lines belonged to a, i and d alleles with 35%, 26% and 21% respectively. The results provided useful information for breeding program to improve breadmaking quality and develop new cultivars.

Keywords

• Glu-1
• Glu-B3
• Wheat