Expression and Purification of Cyanobacterial Circadian Clock Protein KaiC and Determination of Its Auto-phosphatase Activity
Qiang Chen,
Lingling Yu,
Xiao Tan ,
Sen Liu
Affiliations
Qiang Chen
Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, ChinaCollege of Medical Science, China Three Gorges University, Yichang, China
Lingling Yu
College of Medical Science, China Three Gorges University, Yichang, China
Xiao Tan
Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, ChinaCollege of Medical Science, China Three Gorges University, Yichang, China
Sen Liu
Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, ChinaCollege of Medical Science, China Three Gorges University, Yichang, China
Circadian rhythms are biological processes displaying an endogenous oscillation with a period of ~24 h. They allow organisms to anticipate and get prepared for the environmental changes caused mainly by the rotation of Earth. Circadian rhythms are driven by circadian clocks that consist of proteins, DNA, and/or RNA. Circadian clocks of cyanobacteria are the simplest and one of the best studied models. They contain the three clock proteins KaiA, KaiB, and KaiC which can be used for in vitro reconstitution experiments and determination of the auto-phosphatase activity of KaiC as described in this protocol.
