Investigation of The Effects of Trpc4/5 Inhibitor M084 in Experimental Alzheimer’s-LikeDementia Model

Abstract

Purpose: This study aimed to research the effects of N-butyl-1H]-benzimidazole-2-amine (M084) on cognitive functions in an experimental Alzheimer-like dementia model. Materials and Methods: In our study, male rats were divided into five groups (n:8). The control (C) had 1 ml 0.9% saline solution administered intraperitoneally (i.p.) for 14 days. Scopolamine (S) was administered intraperitoneally with 1 ml 0.9% saline solution for the first 7 days, followed by 3 mg/kg scopolamine on days 8-14.Scopolamine+M084 (SM) was administered i.p. dissolved in 1 ml 0.9% saline solution for the first 7 days, followed by 3 mg/kg scopolamine on days 8-14 and 20 mg/kg M084 on days 10-14. M084 (M) was administered i.p. 20 mg/kg M084 in a single daily dose from days 0-14. Positive control (D) had 5 mg/kg donepezil administrations on days 0-14 and 3 mg/kg scopolamine on days 8-14. On days 14 and 15 of the study, passive avoidance, novel object recognition, and modified elevated plus maze tests were performed. Results: In the passive avoidance test, transfer latencies were significantly lower in group S compared to group C. In the modified elevated plus maze test, the passing time to either closed arms on the 2nd-day test was significantly higher in group S compared to groups C and D. In the novel object recognition test, the values for groups C, SM, D, and other groups were significantly higher compared to group S. Conclusion: In conclusion, in an Alzheimer-like dementia model, M084 provided positive results for visual memory; however, it was ineffective for other memory types.

Keywords

• m084
• alzheimer
• scopolamine
• memory functions