A Study on the Biological Characteristics of Kidney Yin Deficiency and Kidney Yang Deficiency Models in SPF-grade Kunming Mice

Abstract

Objective: To investigate the establishment and biological characteristics of Kidney Yin Deficiency (KYD) and Kidney Yang Deficiency (KYD) models in Specific Pathogen Free (SPF)-grade Kunming mice. Methods: Seventy-two SPF-grade Kunming mice were randomly divided into KYD, KYD, and blank control groups. The KYD group received subcutaneous injections of hydrocortisone at 50 mg/kg for 5 days, while the KYD group received 25 mg/kg for 10 days. Various physiological parameters were measured after modeling. Results: Compared to the control group, the KYD and KYD groups showed significant differences in body weight gain, food intake, urine volume, cold water swimming time, grasping irritability, active grid crossings, epididymal sperm count, and active sperm count (P < 0.05). The KYD group had higher isolation-induced aggression scores (P < 0.05), while the KYD group had lower rectal and tail temperatures (P < 0.05). Compared to the KYD group, the KYD group had higher tail and rectal temperatures and increased active grid crossings (P < 0.05). Conclusions: The hydrocortisone-induced KYD and KYD models in mice exhibited corresponding symptoms and biological characteristics, reflecting the features of these syndromes in Traditional Chinese Medicine. This study provides a foundation for further research on Kidney deficiency models.