Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination

Shanshan Wu; Xiyang Liu; Shenglong Ye; Jianmin Liu; Wei Zheng; Xue Dong; Xiushan Yin

Heliyon (Apr 2021)

Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination

Shanshan Wu,
Xiyang Liu,
Shenglong Ye,
Jianmin Liu,
Wei Zheng,
Xue Dong,
Xiushan Yin

Affiliations

Shanshan Wu: Applied Biology Laboratory, College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, 110142, Shenyang, China
Xiyang Liu: Biotech & Biomedicine Science (Shenyang) Co. Ltd, Shenyang, 110000, China
Shenglong Ye: Biotech & Biomedicine Science (Shenyang) Co. Ltd, Shenyang, 110000, China
Jianmin Liu: Shenyang Center for Disease Control and Prevention, 110031, Shenyang, Liaoning, China
Wei Zheng: Liaoning Cancer Hospital and Institute, No. 44 Xiaoheyan Rd, Dadong District, ShenYang, 110042 China
Xue Dong: Shenyang Center for Disease Control and Prevention, 110031, Shenyang, Liaoning, China; Corresponding author.
Xiushan Yin: Applied Biology Laboratory, College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, 110142, Shenyang, China; Biotech & Biomedicine Science (Shenyang) Co. Ltd, Shenyang, 110000, China; SciLifeLab, Department of Microbiology, Tumor and Cell Biology. Karolinska Institutet, Solna 17165, Sweden; Pluri Biotech Co.Ltd, Xuzhou, 221001, China; Nanog Biotech Co.Ltd, Shanghai, 200000, China; Biotech & Biomedicine Science (Jiangxi) Co. Ltd, Ganzhou, 341000, China; Department of Respiratory and Critical Care Medicine, Central Hospital Affiliated to Shenyang Medical College, Shenyang 110024, People's Republic of China; Corresponding author.

Journal volume & issue: Vol. 7, no. 4
p. e06886

Abstract

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RT-LAMP detection of SARS-CoV-2 has been demonstrated to be a valuable diagnostic method for the diagnosis of COVID-191,2, which can rapidly screen carriers of the virus to effectively control the spread of the SARS-CoV-2. Here, we present a combination of dyes for isothermal detection of SARS-CoV-2 as a commercial alternative, with expanded colorimetric spectrum. We compared them with commercial reagents and proved their suitability and sensitivity through clinical RNA samples. In addition, together with commercial single dye indicators, we believe the expanded color spectrum developed here as an indicator of rapid detection will promote the diagnosis of COVID-19.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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