Biotechnology Reports (Dec 2015)

Identification of oxidoreductases from the petroleum Bacillus safensis strain

  • Francine S.A. da Fonseca,
  • Célio F.F. Angolini,
  • Marco A. Zezzi Arruda,
  • Cícero A.L. Junior,
  • Clelton A. Santos,
  • Antonio M. Saraiva,
  • Eduardo Pilau,
  • Anete P. Souza,
  • Prianda R. Laborda,
  • Patrícia F.L. de Oliveira,
  • Valéria M. de Oliveira,
  • Francisco de Assis M. Reis,
  • Anita J. Marsaioli

DOI
https://doi.org/10.1016/j.btre.2015.09.001
Journal volume & issue
Vol. 8, no. C
pp. 152 – 159

Abstract

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A gram-positive bacterium, denominated CFA-06, was isolated from Brazilian petroleum in the Campos Basin and is responsible for the degradation of aromatic compounds and petroleum aromatic fractions. The CFA-06 strain was identified as Bacillus safensis using the 16S rRNA and gyrase B sequence. Enzymatic assays revealed the presence of two oxidoreductases: a catalase and a new oxidoreductase. The oxidoreductases were enzymatically digested and analyzed via ESI-LTQ-Orbitrap mass spectrometry. The mass data revealed a novel oxidoreductase (named BsPMO) containing 224 amino acids and 89% homology with a hypothetic protein from B. safensis (CFA-06) and a catalase (named BsCat) with 491 amino acids and 60% similarity with the catalase from Bacillus pumilus (SAFR-032). The new protein BsPMO contains iron atom(s) and shows catalytic activity toward a monooxygenase fluorogenic probe in the presence of cofactors (NADH, NADPH and NAD). This study enhances our knowledge of the biodegradation process of petroleum by B. safensis.

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